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Anti-ATP5O antibody [4C11C10D12] (ab110276)

MSCatalog No. MS505

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Overview

Product name

Anti-ATP5O antibody [4C11C10D12]
See all ATP5O products (3) ...

Description

Mouse monoclonal [4C11C10D12] to ATP5O

Tested applications

ICC/IF, WB, Flow Cyt, IHC-Pmore details

Cross reactivity

Reacts with

Cow, Human

Immunogen

Recombinant mature Human ATP5O.

Positive control

Isolated mitochondria from Human heart and Bovine heart, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), Human colon tissue, HL-60 cells

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HBS

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

Near homogeneity as judged by SDS-PAGE. ab110276 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Clonality

Monoclonal

Clone number

4C11C10D12

Isotype

IgG1

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex V
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria

  • Western blot - ATP5O antibody [4C11C10D12] (ab110276)Western blot - ATP5O antibody [4C11C10D12] (ab110276) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP5O antibody [4C11C10D12] (ab110276)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP5O antibody [4C11C10D12] (ab110276) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - ATP5O antibody [4C11C10D12] (ab110276)Immunocytochemistry/ Immunofluorescence - ATP5O antibody [4C11C10D12] (ab110276) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab110276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

Sequence similarities

Belongs to the ATPase delta chain family.

Cellular localization

Mitochondrion. Mitochondrion inner membrane.

Target information above from: UniProt accessionP48047 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • mitochondrial antibody
  • ATP synthase O subunit mitochondrial precursor antibody
  • ATP synthase subunit O antibody
  • ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit antibody
  • ATP5O antibody
  • ATPO antibody
  • ATPO_HUMAN antibody
  • EC 3.6.3.14 antibody
  • Mitochondrial ATP synthase, O subunit antibody
  • MS505 antibody
  • Oligomycin sensitivity conferral protein antibody
  • OSCP antibody
see all

Database links

Anti-ATP5O antibody [4C11C10D12] images:

  Western blot - ATP5O antibody [4C11C10D12] (ab110276)

Western blot - ATP5O antibody [4C11C10D12] (ab110276)

All lanes : Anti-ATP5O antibody [4C11C10D12] (ab110276) at 0.5 µg/ml

Lane 1 : Human heart mitochondria at 5 µg
Lane 2 : Bovine heart mitochondria at 1 µg


Predicted band size : 23 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP5O antibody [4C11C10D12] (ab110276)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP5O antibody [4C11C10D12] (ab110276)

Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.

  Immunocytochemistry/ Immunofluorescence - ATP5O antibody [4C11C10D12] (ab110276)

Immunocytochemistry/ Immunofluorescence - ATP5O antibody [4C11C10D12] (ab110276)

Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).

  Flow Cytometry - ATP5O antibody [4C11C10D12] (ab110276)

Flow Cytometry - ATP5O antibody [4C11C10D12] (ab110276)

HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

References for Anti-ATP5O antibody [4C11C10D12] (ab110276)

This product has been referenced in:

  • Sansanwal P  et al. Caspase-4 may play a role in loss of proximal tubules and renal injury in nephropathic cystinosis. Pediatr Nephrol 25:105-9 (2010).Read more (PubMed: 19705160) »
  • Mayr JA  et al. Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 epsilon subunit. Hum Mol Genet 19:3430-9 (2010).Read more (PubMed: 20566710) »

See all 10 publications for this product

Publishing research using ab110276? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"