Anti-ATP5O antibody (ab230325)
Key features and details
- Rabbit polyclonal to ATP5O
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATP5O antibody
See all ATP5O primary antibodies -
Description
Rabbit polyclonal to ATP5O -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Pig, Orangutan -
Immunogen
Synthetic peptide corresponding to Human ATP5O aa 1-100.
Database link: P48047 -
Positive control
- IHC-P:Human skeletal muscle tissue. WB: HEK-293 cell lysate. Flow: HEK-293 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/50.
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WB |
1/1000. Predicted molecular weight: 23 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/50. |
WB
1/1000. Predicted molecular weight: 23 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. -
Sequence similarities
Belongs to the ATPase delta chain family. -
Cellular localization
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: ATP5O Cow
- Entrez Gene: 539 Human
- Omim: 600828 Human
- SwissProt: P13621 Cow
- SwissProt: P48047 Human
- Unigene: 409140 Human
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Alternative names
- ATP synthase O subunit mitochondrial precursor antibody
- ATP synthase subunit O antibody
- ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit antibody
see all
Images
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Anti-ATP5O antibody (ab230325) at 1/1000 dilution + HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 35 µg
Predicted band size: 23 kDa -
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for ATP50 using ab230325 (5 µg/ml) in immunohistochemical analysis.
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Intracellular flow cytometric analysis ofHEK-293 (human epithelial cell line from embryonic kidney) cells labeling ATP50 with ab230325 at 1/10 dilution (green) compared with a negative control (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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All lanes : Anti-ATP5O antibody (ab230325) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : ATP50-transfected HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lysates/proteins at 2 µg per lane.
Predicted band size: 23 kDa -
Paraffin-embedded human skeletal muscle stained for ATP5O (green) using ab230325 (0.05 mg/ml) in immunohistochemical analysis.
FITC-conjugated goat anti-rabbit lgG was used as the secondary antibody. Counterstained with Propidium Iodide (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab230325 has not yet been referenced specifically in any publications.