Anti-ATP6J/ATP6V1G1 antibody (ab220044)
Key features and details
- Rabbit polyclonal to ATP6J/ATP6V1G1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATP6J/ATP6V1G1 antibody -
Description
Rabbit polyclonal to ATP6J/ATP6V1G1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human ATP6J/ATP6V1G1 aa 1 to the C-terminus.
Database link: O75348 -
Positive control
- RT-4 and U-251 MG cell lysates; Human liver lysate; PC-3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 14 kDa.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 14 kDa. |
Target
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Function
Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the V-ATPase G subunit family. - Information by UniProt
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Database links
- Entrez Gene: 281641 Cow
- Entrez Gene: 9550 Human
- Entrez Gene: 66290 Mouse
- Entrez Gene: 298103 Rat
- Omim: 607296 Human
- SwissProt: P79251 Cow
- SwissProt: O75348 Human
- SwissProt: Q9CR51 Mouse
see all -
Alternative names
- ATP 6G antibody
- ATP 6J antibody
- ATP6G antibody
see all
Images
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Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized PC-3 cells labeling ATP6J/ATP6V1G1 with ab220044 at 4 μg/ml (green).
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All lanes : Anti-ATP6J/ATP6V1G1 antibody (ab220044) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Predicted band size: 14 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab220044 has been referenced in 1 publication.
- Maschalidi S et al. UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells. Nat Commun 8:1640 (2017). PubMed: 29158474