Anti-ATPB antibody [7E3F2] (ab110280)
- Product nameAnti-ATPB antibody [7E3F2]See all ATPB primary antibodies ...
- DescriptionMouse monoclonal [7E3F2] to ATPB
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Rat, Cow, Human, Escherichia coli
Native E. coli ATPB.
- Positive controlIsolated mitochondria from Human heart, bovine heart, rat heart, mouse heart, and HepG2 cells.
- Storage instructionsStore at +4°C. Do not freeze.
- Storage bufferPreservative: 0.02% Sodium azide
- Concentration information loading...
- Purity>95% by SDS-PAGE
- Purification notesThe purity of ab110280 is near homogeneity, as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
- Clonality Monoclonal
- Clone number7E3F2
- Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab110280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 4 µg/ml. Predicted molecular weight: 57 kDa.|
- FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
- Sequence similaritiesBelongs to the ATPase alpha/beta chains family.
- Cellular localizationMitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
- ATP 5B antibodyATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibodyATP synthase subunit beta antibody
- ATP synthase subunit beta mitochondrial antibodyATP synthase subunit beta, mitochondrial antibodyatp5b antibodyATPB antibodyATPB_HUMAN antibodyATPMB antibodyATPSB antibodymitochondrial antibodyMitochondrial ATP synthase beta subunit antibodyMitochondrial ATP Synthase Subunit Beta antibodyMitochondrial ATP synthetase beta subunit antibody
Anti-ATPB antibody [7E3F2] images
All lanes : Anti-ATPB antibody [7E3F2] (ab110280) at 4 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 15 µg
Lane 2 : Isolated mitochondria from cow heart at 6 µg
Lane 3 : Isolated mitochondria from rat heart at 30 µg
Lane 4 : Isolated mitochondria from mouse heart at 30 µg
Lane 5 : Isolated mitochondria from HepG2 cells at 30 µg
Predicted band size : 57 kDa
References for Anti-ATPB antibody [7E3F2] (ab110280)
This product has been referenced in:
- Yavlovich A et al. Ectopic ATP synthase facilitates transfer of HIV-1 from antigen-presenting cells to CD4(+) target cells. Blood 120:1246-53 (2012). Read more (PubMed: 22753871) »
- Kim M et al. Increased expression of the F(1)F(o) ATP synthase in response to iron in heart mitochondria. BMB Rep 41:153-7 (2008). WB ; Rat . Read more (PubMed: 18315952) »