Overview

  • Product name
    Anti-ATPB antibody [7E3F2]
    See all ATPB primary antibodies
  • Description
    Mouse monoclonal [7E3F2] to ATPB
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Escherichia coli
  • Immunogen

    Native E. coli ATPB.

  • Positive control
    • Isolated mitochondria from Human heart, bovine heart, rat heart, mouse heart, and HepG2 cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 4 µg/ml. Predicted molecular weight: 57 kDa.

Target

  • Function
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • Sequence similarities
    Belongs to the ATPase alpha/beta chains family.
  • Cellular localization
    Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP 5B antibody
    • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
    • ATP synthase subunit beta mitochondrial antibody
    • ATP synthase subunit beta, mitochondrial antibody
    • atp5b antibody
    • ATPB antibody
    • ATPB_HUMAN antibody
    • ATPMB antibody
    • ATPSB antibody
    • Epididymis secretory protein Li 271 antibody
    • HEL-S-271 antibody
    • Mitochondrial ATP synthase beta subunit antibody
    • Mitochondrial ATP Synthase Subunit Beta antibody
    • Mitochondrial ATP synthetase beta subunit antibody
    see all

Images

  • All lanes : Anti-ATPB antibody [7E3F2] (ab110280) at 4 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 15 µg
    Lane 2 : Isolated mitochondria from cow heart at 6 µg
    Lane 3 : Isolated mitochondria from rat heart at 30 µg
    Lane 4 : Isolated mitochondria from mouse heart at 30 µg
    Lane 5 : Isolated mitochondria from HepG2 cells at 30 µg


    Predicted band size : 57 kDa

References

This product has been referenced in:
  • Yavlovich A  et al. Ectopic ATP synthase facilitates transfer of HIV-1 from antigen-presenting cells to CD4(+) target cells. Blood 120:1246-53 (2012). Read more (PubMed: 22753871) »
  • Kim M  et al. Increased expression of the F(1)F(o) ATP synthase in response to iron in heart mitochondria. BMB Rep 41:153-7 (2008). WB ; Rat . Read more (PubMed: 18315952) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Bacteria Cell lysate - whole cell (Sinorhizobium meliloti whole cell lysate)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
3e+007 cells
Treatment
in SDS sample buffer 2-mercaptoethanol
Specification
Sinorhizobium meliloti whole cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Joseph Chen

Verified customer

Submitted Mar 06 2017

I am very pleased to hear you would like to accept our offer and test ab110280 in Acinetobacter baumannii.. This code will give you one free antibody before the expiration date. To redeem this offer, please submit an Abreview for Acinetobacter baumanni...

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Thank you very much for your interest in ab110280.

To our knowledge, ab110280 has not been tested against samples of Acinetobacter baumannii . Therefore, I can offer a discount off a future purchase if you buy ab110280 now, test it in Acinet...

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Thank you for your email. Yes, we do carry human mitochondrial lysates as well: Human Liver Mitochondrial lysate (ab110339) Human Heart Mitochondrial lysate (ab110337) However, both lysates come in WB loading buffer (ready to be used in WB) ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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