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Overview

  • Product nameAnti-ATPB antibody [7E3F2]See all ATPB primary antibodies ...
  • Description
    Mouse monoclonal [7E3F2] to ATPB
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Escherichia coli
  • Immunogen

    Native E. coli ATPB.

  • Positive controlIsolated mitochondria from Human heart, bovine heart, rat heart, mouse heart, and HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab110280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 4 µg/ml. Predicted molecular weight: 57 kDa.

Target

  • FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • Sequence similaritiesBelongs to the ATPase alpha/beta chains family.
  • Cellular localizationMitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.

    • Target information above from: UniProt accession P06576 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      ATP 5B antibodyATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibodyATP synthase subunit beta antibody
      ATP synthase subunit beta mitochondrial antibodyATP synthase subunit beta, mitochondrial antibodyatp5b antibodyATPB antibodyATPB_HUMAN antibodyATPMB antibodyATPSB antibodymitochondrial antibodyMitochondrial ATP synthase beta subunit antibodyMitochondrial ATP Synthase Subunit Beta antibodyMitochondrial ATP synthetase beta subunit antibody
    see all

Anti-ATPB antibody [7E3F2] images

  • Western blot - ATPB antibody (ab110280)
    Western blot - ATPB antibody (ab110280)
    All lanes : Anti-ATPB antibody [7E3F2] (ab110280) at 4 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 15 µg
    Lane 2 : Isolated mitochondria from cow heart at 6 µg
    Lane 3 : Isolated mitochondria from rat heart at 30 µg
    Lane 4 : Isolated mitochondria from mouse heart at 30 µg
    Lane 5 : Isolated mitochondria from HepG2 cells at 30 µg


    Predicted band size : 57 kDa

References for Anti-ATPB antibody [7E3F2] (ab110280)

This product has been referenced in:
  • Yavlovich A  et al. Ectopic ATP synthase facilitates transfer of HIV-1 from antigen-presenting cells to CD4(+) target cells. Blood 120:1246-53 (2012). Read more (PubMed: 22753871) »
  • Kim M  et al. Increased expression of the F(1)F(o) ATP synthase in response to iron in heart mitochondria. BMB Rep 41:153-7 (2008). WB ; Rat . Read more (PubMed: 18315952) »

See all 2 Publications for this product

Publishing research using ab110280 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

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Votes: Highest First

Sorry for the delayed reply. We would like to proceed and test ab110280 with Acinetobacter baumannii.

I am very pleased to hear you would like to accept our offer and test ab110280 in Acinetobacter baumannii.. This code will give you one free antibody before the expiration date. To redeem this offer, please submit an Abreview for Acinetobacter baumannii a...

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We are interested in an anti-AtpB antibody to detect AtpB of the bacterium Acinetobacter baumannii. We see that you sell an Anti-ATPB antibody [7E3F2] (ab110280) that recognizes Escherichia coli AtpB. Do you know if it reacts with Acinetobacter baumannii ...

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Thank you very much for your interest in ab110280.

To our knowledge, ab110280 has not been tested against samples of Acinetobacter baumannii . Therefore, I can offer a discount off a future purchase if you buy ab110280 now, test it in Acinetoba...

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Thank you very much for the prompt response. I wonder of you also have human mitochondria similar to the bovine preparation? Also, please let me know if the anti ATP synthase that you sell (particularly the mouse monoclonal ab5432 and the monoclonal antob...

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Thank you for your email. Yes, we do carry human mitochondrial lysates as well: Human Liver Mitochondrial lysate (ab110339) Human Heart Mitochondrial lysate (ab110337) However, both lysates come in WB loading buffer (ready to be used in WB) and...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"