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- Secondary antibodies
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Product name
Anti-ATPB antibody [7E3F2]
See all ATPB products (8) ...
Description
Mouse monoclonal [7E3F2] to ATPB
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human, Escherichia coli
Immunogen
Native E. coli ATPB.
Positive control
Isolated mitochondria from Human heart, bovine heart, rat heart, mouse heart, and HepG2 cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
The purity of ab110280 is near homogeneity, as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
7E3F2
Isotype
IgG2a
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex V
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Western blot - ATPB antibody (ab110280)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 4 µg/ml. Predicted molecular weight: 57 kDa.
Target
Function
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Sequence similarities
Belongs to the ATPase alpha/beta chains family.
Cellular localization
Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
Target information above from: UniProt accessionP06576
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- ATP 5B antibody
- ATP synthase subunit beta antibody
see all
Database links
- Entrez Gene: 327675 Cow
- Entrez Gene: 506 Human
- Entrez Gene: 11947 Mouse
- Entrez Gene: 171374 Rat
- Omim: 102910 Human
- SwissProt: P00829 Cow
- SwissProt: P06576 Human
- SwissProt: P56480 Mouse
see all
Anti-ATPB antibody [7E3F2] images:
Western blot - ATPB antibody (ab110280)
All lanes : Anti-ATPB antibody [7E3F2] (ab110280) at 4 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 15 µg
Lane 2 : Isolated mitochondria from cow heart at 6 µg
Lane 3 : Isolated mitochondria from rat heart at 30 µg
Lane 4 : Isolated mitochondria from mouse heart at 30 µg
Lane 5 : Isolated mitochondria from HepG2 cells at 30 µg
Predicted band size : 57 kDa
References for Anti-ATPB antibody [7E3F2] (ab110280)
This product has been referenced in:
- Kim M et al. Increased expression of the F(1)F(o) ATP synthase in response to iron in heart mitochondria. BMB Rep 41:153-7 (2008). WB; Rat.Read more (PubMed: 18315952) »
See 1 publication for this product
Publishing research using ab110280? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"