Loading...
Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
MSCatalog No. MS511
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Anti-ATPB antibody [7E3F2]
See all ATPB products (8) ...
Mouse monoclonal [7E3F2] to ATPB
Reacts with
Mouse, Rat, Cow, Human, Escherichia coli
Native E. coli ATPB.
Isolated mitochondria from Human heart, bovine heart, rat heart, mouse heart, and HepG2 cells.
Liquid
Store at +4°C. Do not freeze.
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration information loading...
>95% by SDS-PAGE
The purity of ab110280 is near homogeneity, as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Monoclonal
7E3F2
IgG2a
kappa
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex V
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Western blot - ATPB antibody (ab110280)
(enlarge)
Our Abpromise guarantee covers the use of ab110280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 4 µg/ml. Predicted molecular weight: 57 kDa.
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Belongs to the ATPase alpha/beta chains family.
Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
Target information above from: UniProt accessionP06576
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - ATPB antibody (ab110280)

All lanes : Anti-ATPB antibody [7E3F2] (ab110280) at 4 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 15 µg
Lane 2 : Isolated mitochondria from cow heart at 6 µg
Lane 3 : Isolated mitochondria from rat heart at 30 µg
Lane 4 : Isolated mitochondria from mouse heart at 30 µg
Lane 5 : Isolated mitochondria from HepG2 cells at 30 µg
Predicted band size : 57 kDa
This product has been referenced in:
See 1 publication for this product
Publishing research using ab110280? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Call 01223 696 000 or contact us
