Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
MS Catalog No. MS990
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All lanes : Anti-ATPG antibody [2A1AA11] (ab119686) at 1 µg/ml
Lane 1 : human heart homogenate lysate at 15 µg
Lane 2 : human HepG2 cell lysate at 15 µg
Lane 3 : human liver mitochondria lysate at 7.5 µg
Lane 4 : rat liver mitochondria lysate at 7.5 µg
Lane 5 : mouse liver mitochondria lysate at 7.5 µg
Lane 6 : bovine heart mitochondria lysate at 7.5 µg
Secondary
Goat anti-mouse HRP at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 33 kDa
Immunocytochemistry using ab119686 stained HDFn cells (human). The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) with antigen retrieval. The cells were then incubated with the antibody (ab119686, 1µg/ml) for 2h at room temperature or over night at 4ºC. The secondary antibody was (red) 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.
Flow cytometry. Hela cells were stained with 1µg/mL anti-ATPG antibody (ab119686) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
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