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Anti-ATPase Inhibitory Factor 1 antibody [5E2D7] (ab110277)

MSCatalog No. MS506

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Overview

Product name

Anti-ATPase Inhibitory Factor 1 antibody [5E2D7]
See all ATPase Inhibitory Factor 1 products (3) ...

Description

Mouse monoclonal [5E2D7] to ATPase Inhibitory Factor 1

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Mouse, Rat, Cow, Human

Immunogen

Recombinant Human ATPase Inhibitory Factor 1 (UniProtKB ID: Q6IAQ7).

Positive control

Isolated mitochondria from Human heart, Bovine heart, Rat heart and Mouse heart

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HBS

Concentration

Concentration information loading...

Purification notes

The purity of ab110277 is near homogeneity, as judged by SDS-PAGE. ab110277 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Clonality

Monoclonal

Clone number

5E2D7

Isotype

IgG1

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex V
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria

  • Western blot - Anti-ATPase Inhibitory Factor 1 antibody [5E2D7] (ab110277)Western blot - Anti-ATPase Inhibitory Factor 1 antibody [5E2D7] (ab110277) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab110277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/ml. Detects a band of approximately 10,18 kDa (predicted molecular weight: 12 kDa).

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    ELISA

     ELISA: Use a concentrati...Read more →

    ELISA: Use a concentration of 4 µg/ml. (In-Cell ELISA, 0.4 µg/well).

Target

Function

Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.

Sequence similarities

Belongs to the ATPase inhibitor family.

Post-translational
modifications

Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.

Cellular localization

Mitochondrion.

Target information above from: UniProt accessionQ9UII2 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • mitochondrial antibody
  • ATIF1_HUMAN antibody
  • ATP synthase inhibitor protein antibody
  • ATPase inhibitor antibody
  • ATPase inhibitor mitochondrial antibody
  • ATPase inhibitor protein antibody
  • ATPase inhibitory factor 1 antibody
  • ATPI antibody
  • ATPIF 1 antibody
  • Atpif1 antibody
  • ATPIP antibody
  • IF(1) antibody
  • IF1 antibody
  • Inhibitor of F(1)F(o)-ATPase antibody
  • IP antibody
  • MGC1167 antibody
  • MGC8898 antibody
  • MS506 antibody
see all

Database links

see all

Anti-ATPase Inhibitory Factor 1 antibody [5E2D7] images:

  Western blot - Anti-ATPase Inhibitory Factor 1 antibody [5E2D7] (ab110277)

Western blot - Anti-ATPase Inhibitory Factor 1 antibody [5E2D7] (ab110277)

All lanes : Anti-ATPase Inhibitory Factor 1 antibody [5E2D7] (ab110277) at 1 µg/ml

Lane 1 : Human heart mitochondria at 10 µg
Lane 2 : Bovine heart mitochondria at 4 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg


Predicted band size : 12 kDa

References for Anti-ATPase Inhibitory Factor 1 antibody [5E2D7] (ab110277)

This product has been referenced in:

  • Cortés-Hernández P  et al. ATP6 homoplasmic mutations inhibit and destabilize the human F1F0-ATP synthase without preventing enzyme assembly and oligomerization. J Biol Chem 282:1051-8 (2007).Read more (PubMed: 17121862) »

See 1 publication for this product

Publishing research using ab110277? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"