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Anti-ATR antibody [2B5] (ab4471)

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Overview

Product name

Anti-ATR antibody [2B5]
See all ATR products (9) ...

Description

Mouse monoclonal [2B5] to ATR

Tested applications

ICC/IF, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR.

Positive control

Raji cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

2B5

Myeloma

NS1

Isotype

IgG

  • Western blot - ATR antibody [2B5] (ab4471)Western blot - ATR antibody [2B5] (ab4471) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab4471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

Tissue specificity

Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.

Involvement in disease

Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.

Sequence similarities

Belongs to the PI3/PI4-kinase family. ATM subfamily.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain.

Post-translational
modifications

Phosphorylated; autophosphorylates in vitro.

Cellular localization

Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.

Target information above from: UniProt accessionQ13535 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ACTR antibody
  • AIB1 antibody
  • amplified in breast cancer 1 antibody
  • Ataxia Telangiecasia and Rad3 Related antibody
  • Ataxia telangiectasia and Rad3-related protein antibody
  • ATR antibody
  • ATR_HUMAN antibody
  • CAGH16 antibody
  • CBP interacting protein antibody
  • CTG26 antibody
  • FRAP Related Protein 1 antibody
  • FRAP-related protein 1 antibody
  • FRP 1 antibody
  • FRP1 antibody
  • Human JTV 1 antibody
  • MEC1 antibody
  • nuclear receptor coactivator 3 antibody
  • pCIP antibody
  • protein kinase ATR antibody
  • RAC3 antibody
  • Rad3 related protein antibody
  • receptor associated coactivator 3 antibody
  • SCKL antibody
  • SCKL1 antibody
  • Seckel syndrome antibody
  • Serine/threonine protein kinase ATR antibody
  • Serine/threonine-protein kinase ATR antibody
  • steroid receptor coactivator protein 3 antibody
  • thyroid hormone receptor activator molecule 1 antibody
  • TRAM1 antibody
see all

Anti-ATR antibody [2B5] images:

  Western blot - ATR antibody [2B5] (ab4471)

Western blot - ATR antibody [2B5] (ab4471)



Predicted band size : 317 kDa


Western blot analysis of ATR protein expression in human Raji whole cell extract.

References for Anti-ATR antibody [2B5] (ab4471)

This product has been referenced in:

  • Blackford ANet al. Adenovirus 12 E4orf6 inhibits ATR activation by promoting TOPBP1 degradation. Proc Natl Acad Sci U S A 107:12251-6 (2010). WB; Human.Read more (PubMed: 20566845) »
  • Ye Jet al. TRF2 and Apollo Cooperate with Topoisomerase 2alpha to Protect Human Telomeres from Replicative Damage. Cell 142:230-242 (2010). ICC/IF; Human.Read more (PubMed: 20655466) »

See all 4 publications for this product

Publishing research using ab4471? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"