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Western blot - ATR antibody [2B5] (ab4471)
Western blot - ATR antibody [2B5] (ab4471)
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Product Name 

ATR antibody [2B5]

 

See all ATR antibodies (7)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [2B5] to ATR

Immunogen 

Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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WB
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Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 317 kDa (predicted molecular weight: 317 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Raji cell lysate.

Cellular localization 

Nuclear

Research areas 

Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR

Relevance 

Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR is a member of the DNA-PK kinases closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes. Several known components of the NuRD complex including histone deacetylase 1 (HDAC1), HDAC2, and CHD4.

 

Alternative names ATR antibody [2B5] (ab4471)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

545    (Human)

Omim

    

601215    (Human)

SwissProt

    

Q13535    (Human)

Unigene

    

271791    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

2B5

Myeloma 

NS1

Isotype 

IgG

Purity 

Protein G purified

Storage buffer 

Preservative: None
Constituents: PBS, pH 7.2

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATR antibody [2B5] is on this datasheet. But please do contact us if you would like any reassurance!


See below for ATR antibody [2B5] images, references, products related to ab4471 and other tools.

 

ATR antibody [2B5] images:

Product image  Western blot - ATR antibody [2B5] (ab4471)

Western blot - ATR antibody [2B5] (ab4471)



Predicted band size : 317 kDa


Western blot analysis of ATR protein expression in human Raji whole cell extract.

 

References for ATR antibody [2B5] (ab4471)

Specific publication references   This product has been used in: (two most recent references)

Blackford AN et al. A role for E1B-AP5 in ATR signaling pathways during adenovirus infection. J Virol 82:7640-52 (2008). WB; Human. PubMed: 18480432

Post S et al. Phosphorylation of serines 635 and 645 of human Rad17 is cell cycle regulated and is required for G(1)/S checkpoint activation in response to DNA damage. Proc Natl Acad Sci U S A 98:13102-7 (2001). PubMed: 11687627
If you publish research using ab4471 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to ATR

 

ATR antibody [2B5] - more information

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Price and availability of products related to ATR antibody [2B5]

 

ATR antibody [2B5] - related products:

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ATR antibody [2B5] - other tools:

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