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Western blot - ATR antibody [2B5] (ab4471)
![Western blot - ATR antibody [2B5] (ab4471)](/ps/datasheet/images/ab4471.jpg)
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Product Name
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ATR antibody [2B5]
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See all ATR antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [2B5] to ATR
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Immunogen
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Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 317 kDa (predicted molecular weight: 317 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Raji cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
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Relevance
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Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR is a member of the DNA-PK kinases closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes. Several known components of the NuRD complex including histone deacetylase 1 (HDAC1), HDAC2, and CHD4.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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2B5
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Myeloma
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NS1
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATR antibody [2B5] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ATR antibody [2B5] images, references, products related to ab4471 and other tools.
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ATR antibody [2B5] images:
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 Western blot - ATR antibody [2B5] (ab4471)
Predicted band size : 317 kDa
Western blot analysis of ATR protein expression in human Raji whole cell extract.
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References for ATR antibody [2B5] (ab4471)
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This product has been used in: (two most recent references)
Blackford AN et al. A role for E1B-AP5 in ATR signaling pathways during adenovirus infection. J Virol 82:7640-52 (2008). WB; Human. PubMed: 18480432
Post S et al. Phosphorylation of serines 635 and 645 of human Rad17 is cell cycle regulated and is required for G(1)/S checkpoint activation in response to DNA damage. Proc Natl Acad Sci U S A 98:13102-7 (2001). PubMed: 11687627
If you publish research using ab4471 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ATR
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ATR antibody [2B5] - more information
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Customer reviews (feedback) regarding ATR antibody [2B5] |
Customer FAQs regarding ATR antibody [2B5] |
Protocols for ATR antibody [2B5] |
Price and availability of products related to ATR antibody [2B5] |
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ATR antibody [2B5] - related products:
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ATR antibody [2B5] - other tools:
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Contact Abcam with a Technical Enquiry about ATR antibody [2B5] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - ATR antibody [2B5] (ab4471)](/index.html?datasheet=4471#ab4471.jpg)
