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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
Overview
Product name
Anti-ATR antibody
See all ATR products (9) ...
Description
Rabbit polyclonal to ATR
Specificity
We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Immunogen was a synthetic peptide, which represented a portion of human Ataxia and Rad3 related encoded within exon 5 (LocusLink ID 545).
Positive control
Whole cell lysate from HeLa and U2OS cells
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
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Western blot - ATR antibody (ab10312)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab10312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: 1/15000 - 1/30000. Detects a band of approximately 300 kDa (predicted molecular weight: 317 kDa). Block membrane with 5% milk and incubate antibodies (primary and secondary) in milk.
This antibody can often recognise extra bands which are fragments of ATR.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
Tissue specificity
Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
Involvement in disease
Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
Sequence similarities
Belongs to the PI3/PI4-kinase family. ATM subfamily.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain.
Post-translational
modifications
Phosphorylated; autophosphorylates in vitro.
Cellular localization
Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
Target information above from: UniProt accessionQ13535
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACTR antibody
- AIB1 antibody
- amplified in breast cancer 1 antibody
see all
Database links
- Entrez Gene: 545 Human
- Entrez Gene: 245000 Mouse
- Omim: 601215 Human
- SwissProt: Q13535 Human
- SwissProt: Q9JKK8 Mouse
- Unigene: 271791 Human
- Unigene: 212462 Mouse
Anti-ATR antibody images:
Western blot - ATR antibody (ab10312)
Predicted band size : 317 kDa
Detection of human ATR by Western Blot. Samples: Whole cell lysate (20 µg) from HeLa and U2OS cells separated on a 3 to 8% tris-acetate gel. Antibody: ab10312 used at 0.07 mg/ml. Detection: Chemiluminescence with 15 second exposure.
References for Anti-ATR antibody (ab10312)
This product has been referenced in:
- Treanor Let al. Independent regulation of P53 stabilisation and activation after Rb deletion in primary epithelial cells. Int J Oncol 37:31-9 (2010). ICC/IF, WB; Mouse.Read more (PubMed: 20514394) »
- Derheimer FAet al. RPA and ATR link transcriptional stress to p53. Proc Natl Acad Sci U S A 104:12778-83 (2007).Read more (PubMed: 17616578) »
See all 2 publications for this product
Publishing research using ab10312? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"