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Western blot - ATR antibody (ab10312)
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Product Name
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ATR antibody
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See all ATR antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ATR
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Immunogen
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Immunogen was a synthetic peptide, which represented a portion of human Ataxia and Rad3 related encoded within exon 5 (LocusLink ID 545).
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Reacts with
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Hu, Ms
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Does not react with
(species key)
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Xl
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/15000 - 1/30000. Detects a band of approximately 300 kDa (predicted molecular weight: 317 kDa). Block membrane with 5% milk and incubate antibodies (primary and secondary) in milk.
This antibody can often recognise extra bands which are fragments of ATR.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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Whole cell lysate from HeLa and U2OS cells
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
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Relevance
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Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR is a member of the DNA-PK kinases closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes. Several known components of the NuRD complex including histone deacetylase 1 (HDAC1), HDAC2, and CHD4.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: Tris-citrate/phosphate. pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATR antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ATR antibody images, references, products related to ab10312 and other tools.
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ATR antibody images:
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 Western blot - ATR antibody (ab10312)
Predicted band size : 317 kDa
Detection of human ATR by Western Blot. Samples: Whole cell lysate (20 µg) from HeLa and U2OS cells separated on a 3 to 8% tris-acetate gel. Antibody: ab10312 used at 0.07 mg/ml. Detection: Chemiluminescence with 15 second exposure.
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References for ATR antibody (ab10312)
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This product has been used in:
Derheimer FA et al. RPA and ATR link transcriptional stress to p53. Proc Natl Acad Sci U S A 104:12778-83 (2007). PubMed: 17616578
If you publish research using ab10312 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ATR
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ATR antibody - more information
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Customer reviews (feedback) regarding ATR antibody |
Customer FAQs regarding ATR antibody |
Protocols for ATR antibody |
Price and availability of products related to ATR antibody |
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ATR antibody - related products:
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ATR antibody - other tools:
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Contact Abcam with a Technical Enquiry about ATR antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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