Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
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ab2905 has been referenced in 7 publications.
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All lanes : Anti-ATR antibody - ChIP Grade (ab2905) at 1/1000 dilution
Lane 1 : MCF10A breast epithelial cells, grown in normoxic conditions (20% O2). Cells were lysed with NP40.
Lane 2 : MCF10A Breast Epithelial cells grown in hypoxic. conditions (5% O2). Cells were lysed with NP40.
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti Rabbit (HRP) at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 309 kDa
Observed band size : 300 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Blocking: Carried out using non fat dry milk for 1 hr at room temperature.
This image is an edited version of an image submitted courtesy of an Abreview by Francisco Ramirez-Valle submitted on 15th November 2005. We do not have any further information relating to this image.
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