Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
Overview
Product name
Anti-ATR antibody - ChIP Grade
See all ATR products (9) ...
Description
Rabbit polyclonal to ATR - ChIP Grade
Tested applications
IF, IP, WB, ChIPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Fusion protein, corresponding to amino acids 400-460 of Human ATR.
Positive control
UV irradiated K562 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: Use at an assay dependent dilution.
IP: Use a concentration of 2 mg/ml
WB: 1/1000Detects a band of approximately 305 kDa (predicted molecular weight: 309 kDa).( (in lysate from UV irradiated K562 cells).)
ChIP: Use at an assay dependent dilution. (PubMed: 20647539)
Target
Function
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
Tissue specificity
Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
Involvement in disease
Defects in ATR are a cause of Seckel syndrome type 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation, microcephaly with mental retardation, and a characteristic 'bird-headed' facial appearance.
Sequence similarities
Belongs to the PI3/PI4-kinase family. ATM subfamily.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain.
Post-translational
modifications
Phosphorylated; autophosphorylates in vitro.
Cellular localization
Nucleus. Nucleus > PML body. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
Target information above from: UniProt accessionQ13535
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACTR antibody
- AIB1 antibody
- amplified in breast cancer 1 antibody
see all
Database links
- Entrez Gene: 545 Human
- Entrez Gene: 245000 Mouse
- Omim: 601215 Human
- SwissProt: Q13535 Human
- SwissProt: Q9JKK8 Mouse
- Unigene: 271791 Human
- Unigene: 212462 Mouse
Anti-ATR antibody - ChIP Grade images:
Western blot - ATR antibody (ab2905)
All lanes : Anti-ATR antibody - ChIP Grade (ab2905) at 1/1000 dilution
Lane 1 : MCF10A breast epithelial cells, grown in normoxic conditions (20% O2). Cells were lysed with NP40.
Lane 2 : MCF10A Breast Epithelial cells grown in hypoxic. conditions (5% O2). Cells were lysed with NP40.
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti Rabbit (HRP) at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 309 kDa
Observed band size : 300 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Blocking: Carried out using non fat dry milk for 1 hr at room temperature.
This image is an edited version of an image submitted courtesy of an Abreview by Francisco Ramirez-Valle submitted on 15th November 2005. We do not have any further information relating to this image.
References for Anti-ATR antibody - ChIP Grade (ab2905)
This product has been referenced in:
- Lin Yet al. Convergent transcription through a long CAG tract destabilizes repeats and induces apoptosis. Mol Cell Biol 30:4435-51 (2010). ChIP; Human.Read more (PubMed: 20647539) »
- Siehler SYet al. Human MutL-complexes monitor homologous recombination independently of mismatch repair. DNA Repair (Amst) 8:242-52 (2009).Read more (PubMed: 19022408) »
See all 6 publications for this product
Publishing research using ab2905? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"