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Anti-ATRIP (phospho S68 + S72) antibody (ab63380)

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Overview

Product name

Anti-ATRIP (phospho S68 + S72) antibody
See all ATRIP products (5) ...

Description

Rabbit polyclonal to ATRIP (phospho S68 + S72)

Specificity

Thid antibody detects endogenous levels of ATRIP only when phosphorylated at serine 68/72 (human) and serine 69/73 (mouse and rat).

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic phosphopeptide from around the phosphorylation site of serine 68/72 (LASPQA) of human ATRIP.

Positive control

NIH/3T3 cell extract

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR

  • Western blot - ATRIP (phospho S68 + S72) antibody (ab63380)Western blot - ATRIP (phospho S68 + S72) antibody (ab63380) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab63380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The product of the ATRIP gene is an essential component of the DNA damage checkpoint, and binds to single stranded DNA coated with replication protein A that accumulates at sites of DNA damage. The encoded protein interacts with the ataxia telangiectasia and Rad3 related protein, a checkpoint kinase, resulting in accumulation of the kinase at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.

Cellular localization

Nuclear

Alternative names

  • AGS1 antibody
  • ATM and Rad3 related interacting protein antibody
  • ATR interacting protein antibody
  • TREX1 antibody

Database links

Anti-ATRIP (phospho S68 + S72) antibody images:

  Western blot - ATRIP (phospho S68 + S72) antibody (ab63380)

Western blot - ATRIP (phospho S68 + S72) antibody (ab63380)

All lanes : Anti-ATRIP (phospho S68 + S72) antibody (ab63380) at 1/500 dilution

Lane 1 : NIH/3T3 cell extract
Lane 2 : NIH/3T3 cell extract with immunising phosphopeptide at 10 µg

Lysates/proteins at 30 µg per lane.


Predicted band size : 86 kDa
Observed band size : 86 kDa

References for Anti-ATRIP (phospho S68 + S72) antibody (ab63380)

ab63380 has not yet been referenced specifically in any publications.

Publishing research using ab63380? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"