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Western blot - ATRIP (phospho S68 + S72) antibody (ab63380)
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Product Name
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ATRIP (phospho S68 + S72) antibody
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See all ATRIP antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ATRIP (phospho S68 + S72)
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Immunogen
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Synthetic phosphopeptide from around the phosphorylation site of serine 68/72 (LASPQA) of human ATRIP.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Thid antibody detects endogenous levels of ATRIP only when phosphorylated at serine 68/72 (human) and serine 69/73 (mouse and rat).
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/40000.
WB: 1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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NIH/3T3 cell extract
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> ATM / ATR
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Relevance
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The product of the ATRIP gene is an essential component of the DNA damage checkpoint, and binds to single stranded DNA coated with replication protein A that accumulates at sites of DNA damage. The encoded protein interacts with the ataxia telangiectasia and Rad3 related protein, a checkpoint kinase, resulting in accumulation of the kinase at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against
non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ATRIP (phospho S68 + S72) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ATRIP (phospho S68 + S72) antibody images, references, products related to ab63380 and other tools.
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ATRIP (phospho S68 + S72) antibody images:
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 Western blot - ATRIP (phospho S68 + S72) antibody (ab63380)
All lanes : ATRIP (phospho S68 + S72) antibody (ab63380) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract
Lane 2 : NIH/3T3 cell extract with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 86 kDa
Observed band size : 86 kDa
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Search PubMed (MEDLINE) for references to ATRIP
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ATRIP (phospho S68 + S72) antibody - more information
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ATRIP (phospho S68 + S72) antibody - related products:
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ATRIP (phospho S68 + S72) antibody - other tools:
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