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All lanes : Anti-ATRX antibody (ab72124) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size : 283 kDa
Observed band size : 283 kDa
A range of bands are detected from 117-460 kDa.
Immunoprecipitation/ Western Blot of ATRX.
Lane 1: ab72124 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72124 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 10 seconds.
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