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Products:Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Swi / Snf
Overview
Product name
Anti-ATRX antibody
See all ATRX products (6) ...
Description
Rabbit polyclonal to ATRX
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residue 2442 and the C-terminus (residue 2492) of human ATRX (NP_000480.2).
Positive control
Whole cell lysate from Hela cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Swi / Snf
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 283 kDa and various other bands ranging from 117 kDa to 460 kDa (predicted molecular weight: 283 kDa).
Note: Best results were obtained using 4% Tris-Glycine gels and transferring to nitrocellulose for a minimum of 5 hours.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Could be a global transcriptional regulator. Modifies gene expression by affecting chromatin. May be involved in brain development and facial morphogenesis.
Tissue specificity
Ubiquitous.
Involvement in disease
Defects in ATRX are the cause of alpha-thalassemia mental retardation syndrome X-linked non-deletion type (ATRX) [MIM:301040]. ATR-X is an X-linked disorder comprising severe psychomotor retardation, facial dysmorphism, urogenital abnormalities, and alpha-thalassemia. An essential phenotypic trait are hemoglobin H erythrocyte inclusions.
Defects in ATRX are the cause of mental retardation syndromic X-linked with hypotonic facies syndrome type 1 (MRXSHF1) [MIM:309580]; also called Carpenter-Waziri syndrome (CWS), Juberg-Marsidi syndrome (JMS), Smith-Fineman-Myers syndrome type 1 (SFM1). Clinical features include severe mental retardation, dysmorphic facies, and a highly skewed X-inactivation pattern in carrier women. Other more variable features include hypogonadism, deafness, renal anomalies, and mild skeletal defects.
Defects in ATRX are a cause of alpha-thalassemia myelodysplasia syndrome (ATMDS) [MIM:300448]. In this disorder, alpha-thalassemia occurs as an acquired abnormality in association with a multilineage myelodysplasia.
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 PHD-type zinc finger.
Domain
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. Associated with pericentromeric heterochromatin during interphase and mitosis, probably by interacting with HP1.
Target information above from: UniProt accessionP46100
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha thalassemia/mental retardation syndrome X linked homolog antibody
- ATP dependent helicase ATRX antibody
- ATP-dependent helicase ATRX antibody
see all
Database links
- Entrez Gene: 546 Human
- Omim: 301040 Human
- SwissProt: P46100 Human
- Unigene: 533526 Human
- Unigene: 653797 Human
Anti-ATRX antibody images:
Western blot - ATRX antibody (ab72124)
All lanes : Anti-ATRX antibody (ab72124) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size : 283 kDa
Observed band size : 283 kDa
A range of bands are detected from 117-460 kDa.
Immunoprecipitation - ATRX antibody (ab72124)
Immunoprecipitation/ Western Blot of ATRX.
Lane 1: ab72124 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72124 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 10 seconds.
References for Anti-ATRX antibody (ab72124)
ab72124 has not yet been referenced specifically in any publications.
Publishing research using ab72124? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"