Overview

  • Product nameAnti-ATRX antibody
    See all ATRX primary antibodies
  • Description
    Rabbit polyclonal to ATRX
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Tammar wallaby (Macropus eugenii)
  • Immunogen

    Synthetic peptide corresponding to Human ATRX aa 2442-2492 (C terminal).
    Database link: P46100

  • Positive control
    • human Breast Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Prostate Carcinoma, Skin Basal Cell Carcinoma, Stomach Adenocarcinoma tissue.
  • General notes

    Concentration is optimized for IHC and not determined

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibody was affinity purified using an epitope specific to ATRX immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.

Target

  • FunctionInvolved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells which probably implies recruitment of CBX5 to telomers. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting; according to a report, it is not sufficient to decrease chromatin condensation at telomers nor to increase expression of telomeric RNA in fibroblasts (PubMed:24500201). May be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. Acts as negative regulator of chromatin incorporation of transcriptionally repressive histone H2AFY, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. May be involved in brain development and facial morphogenesis. Binds to zinc-finger coding genes with atypical chromatin signatures and regulates its H3K9me3 levels. Forms a complex with ZNF274, TRIM28 and SETDB1 to facilitate the deposition and maintenance of H3K9me3 at the 3' exons of zinc-finger genes (PubMed:27029610).
  • Tissue specificityUbiquitous.
  • Involvement in diseaseAlpha-thalassemia mental retardation syndrome, X-linked
    Mental retardation, X-linked, syndromic, with hypotonic facies 1
    Alpha-thalassemia myelodysplasia syndrome
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PHD-type zinc finger.
  • DomainThe ADD domain predominantly interacts with histone H3 trimethylated at 'Lys-10'(H3K9me3) (and to a lesser extent H3 mono-or dimethylated at 'Lys-10') and simultanously to histone H3 unmethylated at 'Lys-5' (H3K4me0). The interaction with H3K9me3 is disrupted by the presence of H3K4me3 suggesting a readout of the combined histone H3 methylation state.
    Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
  • Post-translational
    modifications
    Phosphorylated at serine residues during mitose. Phosphorylation may promote the release from the nuclear matrix and progression to mitosis.
  • Cellular localizationNucleus. Chromosome, telomere. Nucleus, PML body. Associated with pericentromeric heterochromatin during interphase and mitosis, probably by interacting with CBX5/HP1 alpha. Colocalizes with histone H3.3, DAXX, HIRA and ASF1A at PML-nuclear bodies. Colocalizes with cohesin (SMC1 and SMC3) and MECP2 at the maternal H19 ICR (By similarity).
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha thalassemia/mental retardation syndrome X linked homolog antibody
    • ATP dependent helicase ATRX antibody
    • ATP-dependent helicase ATRX antibody
    • ATR2 antibody
    • Atrx antibody
    • ATRX_HUMAN antibody
    • DNA dependent ATPase and helicase antibody
    • Helicase 2, X linked antibody
    • MGC2094 antibody
    • MRXHF1 antibody
    • RAD54 antibody
    • RAD54L antibody
    • SFM1 antibody
    • SHS antibody
    • Transcriptional regulator ATRX antibody
    • X linked helicase II antibody
    • X linked nuclear protein antibody
    • X-linked helicase II antibody
    • X-linked nuclear protein antibody
    • XH2 antibody
    • XNP antibody
    • Znf HX antibody
    • Znf-HX antibody
    see all

Anti-ATRX antibody images

  • ab84462, at 1/250 dilution, staining ATRX in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry, using DAB detectioN.
  • ab84462, at 1/250 dilution, staining ATRX in formalin-fixed, paraffin-embedded human basal cell carcinoma by Immunohistochemistry, using DAB detection.

References for Anti-ATRX antibody (ab84462)

ab84462 has not yet been referenced specifically in any publications.

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