Overview

  • Product nameAnti-ATRX antibodySee all ATRX primary antibodies ...
  • Description
    Rabbit polyclonal to ATRX
  • Tested applicationsIHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Tammar wallaby (Macropus eugenii)
  • Immunogen

    Synthetic peptide, corresponding to a region between C terminal amino acids 2442-2492 of Human ATRX (NP_000480.2).

  • Positive control
    • human Breast Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Prostate Carcinoma, Skin Basal Cell Carcinoma, Stomach Adenocarcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibody was affinity purified using an epitope specific to ATRX immobilized on solid support.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab84462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCould be a global transcriptional regulator. Modifies gene expression by affecting chromatin. May be involved in brain development and facial morphogenesis.
    • Tissue specificityUbiquitous.
    • Involvement in diseaseDefects in ATRX are the cause of alpha-thalassemia mental retardation syndrome X-linked non-deletion type (ATRX) [MIM:301040]. ATR-X is an X-linked disorder comprising severe psychomotor retardation, facial dysmorphism, urogenital abnormalities, and alpha-thalassemia. An essential phenotypic trait are hemoglobin H erythrocyte inclusions.
      Defects in ATRX are the cause of mental retardation syndromic X-linked with hypotonic facies syndrome type 1 (MRXSHF1) [MIM:309580]; also called Carpenter-Waziri syndrome (CWS), Juberg-Marsidi syndrome (JMS), Smith-Fineman-Myers syndrome type 1 (SFM1). Clinical features include severe mental retardation, dysmorphic facies, and a highly skewed X-inactivation pattern in carrier women. Other more variable features include hypogonadism, deafness, renal anomalies, and mild skeletal defects.
      Defects in ATRX are a cause of alpha-thalassemia myelodysplasia syndrome (ATMDS) [MIM:300448]. In this disorder, alpha-thalassemia occurs as an acquired abnormality in association with a multilineage myelodysplasia.
    • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
      Contains 1 ADD domain.
      Contains 1 GATA-type zinc finger.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 PHD-type zinc finger.
    • DomainContains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus. Associated with pericentromeric heterochromatin during interphase and mitosis, probably by interacting with HP1.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha thalassemia/mental retardation syndrome X linked homolog antibody
      • ATP dependent helicase ATRX antibody
      • ATP-dependent helicase ATRX antibody
      • ATR2 antibody
      • Atrx antibody
      • ATRX_HUMAN antibody
      • DNA dependent ATPase and helicase antibody
      • Helicase 2, X linked antibody
      • MGC2094 antibody
      • MRXHF1 antibody
      • RAD54 antibody
      • RAD54L antibody
      • SFM1 antibody
      • SHS antibody
      • Transcriptional regulator ATRX antibody
      • X linked helicase II antibody
      • X linked nuclear protein antibody
      • X-linked helicase II antibody
      • X-linked nuclear protein antibody
      • XH2 antibody
      • XNP antibody
      • Znf HX antibody
      • Znf-HX antibody
      see all

    Anti-ATRX antibody images

    • ab84462, at 1/250 dilution, staining ATRX in formalin-fixed, paraffin-embedded human breast carcinoma by Immunohistochemistry, using DAB detectioN.
    • ab84462, at 1/250 dilution, staining ATRX in formalin-fixed, paraffin-embedded human basal cell carcinoma by Immunohistochemistry, using DAB detection.

    References for Anti-ATRX antibody (ab84462)

    ab84462 has not yet been referenced specifically in any publications.

    Product Wall

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