Recombinant Anti-ATTY antibody [EPR6121] (ab125000)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6121] to ATTY
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ATTY antibody [EPR6121]
See all ATTY primary antibodies -
Description
Rabbit monoclonal [EPR6121] to ATTY -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human ATTY aa 300-400. The exact sequence is proprietary.
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Positive control
- HeLa whole cell lysate (ab29545) can be used as a positive control in WB. HepG2 (treated with insulin), HepG2, and A549 cell lysates; Human brain tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.19 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6121 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
Target
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Function
Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has no transaminase activity towards phenylalanine. -
Pathway
Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 2/6. -
Involvement in disease
Defects in TAT are the cause of tyrosinemia type 2 (TYRO2) [MIM:276600]; also known as Richner-Hanhart syndrome. TYRO2 is an inborn error of metabolism characterized by elevations of tyrosine in the blood and urine, and oculocutaneous manifestations. Typical features include palmoplantar keratosis, painful corneal ulcers, and mental retardation. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. - Information by UniProt
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Database links
- Entrez Gene: 6898 Human
- Entrez Gene: 234724 Mouse
- Entrez Gene: 24813 Rat
- Omim: 613018 Human
- SwissProt: P17735 Human
- SwissProt: Q8QZR1 Mouse
- SwissProt: P04694 Rat
- Unigene: 161640 Human
see all -
Alternative names
- ATTY_HUMAN antibody
- L-tyrosine:2-oxoglutarate aminotransferase antibody
- TAT antibody
see all
Images
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All lanes : Anti-ATTY antibody [EPR6121] (ab125000) at 1/1000 dilution
Lane 1 : HepG2 cell lysate, treated with insulin
Lane 2 : HepG2 cell lysate, untreated
Lane 3 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 50 kDa -
ab125000 at 1/50 dilution staining ATTY in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab125000 has been referenced in 1 publication.
- Nandi SS et al. Lack of miR-133a Decreases Contractility of Diabetic Hearts: A Role for Novel Cross Talk Between Tyrosine Aminotransferase and Tyrosine Hydroxylase. Diabetes 65:3075-90 (2016). PubMed: 27411382