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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Centrosome
Overview
Product name
Anti-AZI1 antibody
See all AZI1 products (5) ...
Description
Rabbit polyclonal to AZI1
Tested applications
ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, corresponding to a region between residue 1033 and 1083 of human AZI1 (EAW89632.1)
Positive control
HeLa Cells.
General notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified using an epitope specific to AZI1 immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Centrosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
ICC/IF
Application notes
ICC/IF: 1/100 - 1/500. Formaldehyde-Fixed or Acetone fixed cells.
Permeabilization with Triton-X 100 is recommended.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May play a role in spermatogenesis.
Sequence similarities
Contains 1 IQ domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm > cytoskeleton > centrosome.
Target information above from: UniProt accessionQ9UPN4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5 azacytidine induced 1 antibody
- Centrosomal protein of 131 kDa antibody
- Cep131 antibody
see all
Database links
- Entrez Gene: 22994 Human
- Omim: 613479 Human
- SwissProt: Q9UPN4 Human
- Unigene: 514578 Human
Anti-AZI1 antibody images:
Immunocytochemistry/ Immunofluorescence - AZI1 antibody (ab84864)
ab84864 at 1/250 dilution staining AZI1 in asynchronous HeLa cells by Immunocytochemistry, NBF-fixed. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
Immunocytochemistry/ Immunofluorescence - AZI1 antibody (ab84864)
ab84864 at 1/250 dilution staining AZI1 in asynchronous HeLa cells by Immunocytochemistry, NBF-fixed. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
References for Anti-AZI1 antibody (ab84864)
ab84864 has not yet been referenced specifically in any publications.
Publishing research using ab84864? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"