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Acetoacetyl CoA synthetase protein (His-DHFR tag) (ab127226)

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Overview

  • Product nameAcetoacetyl CoA synthetase protein (His-DHFR tag)
  • Protein descriptionRecombinant fragment of Human Acetoacetyl CoA synthetase (amino acids 384-601) with an N terminal His-DHFR tag, predicted mwt: 24.5 kDa without the tag (BC051862; UniProt Q86V21).
  • Uniprot accessionQ86V21
  • Molecular weight24.500kDa
  • Protein length218 amino acids
  • Expression hostE. coli

    • Properties

  • Purification notesPurity was determined to be >95% by SDS-PAGE.
  • E.C. Number6.2.1.16
  • FormLyophilised:Reconstitute with water to desired concentration.
  • Storage instructionsShipped at 4°C. Store at -20ºC.
  • Storage bufferConstituents: 0.32% Tris HCl, 0.58% Sodium chloride
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab127226 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        AacsAACS_HUMANAcetoacetate CoA ligase
        Acetoacetyl-CoA synthetaseACSF 1ACSF1Acyl CoA synthetase family member 1Acyl-CoA synthetase family member 1FLJ12389FLJ41251Homolog of C. elegans supressor of ras 5Homolog of C. elegans supressor of ras 5 (sur 5)Protein sur 5 homologProtein sur-5 homologSUR 5SUR5
      see all
  • FunctionActivates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development.
  • Tissue specificityHighly expressed in kidney, heart and brain, but low in liver.
  • Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
  • Cellular localizationCytoplasm > cytosol.

    • Target information above from: UniProt accession Q86V21 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Acetoacetyl CoA synthetase protein (His-DHFR tag) (ab127226)

    ab127226 has not yet been referenced specifically in any publications.

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