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Western blot - Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
Western blot - Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
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Immunohistochemistry (Paraffin-embedded sections) - Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
Immunohistochemistry (Paraffin-embedded sections) - Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
(enlarge)


Product Name 

Acetyl Coenzyme A Carboxylase antibody [EP687Y]

 

See all Acetyl Coenzyme A Carboxylase antibodies (6)...

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP687Y] to Acetyl Coenzyme A Carboxylase

Immunogen 

Synthetic peptide corresponding to residues in human Acetyl Coenzyme A Carboxylase.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
IHC-P: 1/50 - 1/100. Antigen retrieval recommended.
WB: 1/2000. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).

Is unsuitable for FACS, ICC/IF or IP.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A431 cell lysate, human liver tissue.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis

Relevance 

In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl CoA. In fatty acid synthesis, catalytic formation of malonyl CoA (precursor for long chain fatty acyl CoA, LCFA CoA) from acetyl CoA by Acetyl CoA carboxylase (ACC1) is the rate limiting step. The translocation of LCFA CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT1 and CPT2) and regulated by ACC2, the rate limiting step of mitochondrial fatty acid beta oxidation. Activities of ACC1 and 2 are regulated by their phosphorylation by 5' AMP activated protein kinase (AMPK). Diabetes deranges AMPK master switch and represses the ACC1 gene expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC1 is also known as ACC alpha is a cytosolic enzyme, enriched in liver, adipose and lactating mammary tissues.
ACC1 catalyzes the carboxylation of acetyl CoA to form malonyl CoA, the rate limiting step in the biogenesis of LCFA CoA. ACC1 carries three functions: biotin carboxyl carrier protein, biotin carboxylase, and carboxyltransferase (catalytic activity). Two variants of ACC1 have been described. One with 8 additional amino acids commencing at Pro 1196. The other which is 59aa shorter than the predominant fat and liver isoform exist in mammals. The presence of 8 additional amino acids inhibits the in vitro phosphorylation of the Ser1200 by cAMP dependent kinase. The two ACC1 isoform are differentially regulated in a tissue specific manner and under different physiological conditions. The activity of ACC1 is finely regulated by hormone dependent phosphorylation and dephosphorylation.

 

Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

31    (Human)
32    (Human)
107476    (Mouse)
100705    (Mouse)
116719    (Rat)
60581    (Rat)

Omim

    

200350    (Human)
601557    (Human)

SwissProt

    

Q13085    (Human)
O00763    (Human)
Q5SWU9    (Mouse)
P11497    (Rat)
O70151    (Rat)

Unigene

    

234898    (Human)
160556    (Human)
44372    (Rat)

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP687Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Acetyl Coenzyme A Carboxylase antibody [EP687Y] is on this datasheet. But please do contact us if you would like any reassurance!


See below for Acetyl Coenzyme A Carboxylase antibody [EP687Y] images, references, products related to ab45174 and other tools.

 

Acetyl Coenzyme A Carboxylase antibody [EP687Y] images:

Product image  Western blot - Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)

Western blot - Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)

Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174) at 1/2000 dilution + A431 cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP labelled, at 1/2000 dilution

Predicted band size : 265 kDa
Observed band size : 265 kDa

 

Product image  Immunohistochemistry (Paraffin-embedded sections) - Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)

Immunohistochemistry (Paraffin-embedded sections) - Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)

Ab45174, at a dilution of 1/50, staining human Acetyl Coenzyme A Carboxylase in human liver by immunohistochemistry using paraffin embedded tissue.

 
 

Search PubMed (MEDLINE) for references to Acetyl Coenzyme A Carboxylase

 

Acetyl Coenzyme A Carboxylase antibody [EP687Y] - more information

Customer reviews (feedback) regarding Acetyl Coenzyme A Carboxylase antibody [EP687Y]

Customer FAQs regarding Acetyl Coenzyme A Carboxylase antibody [EP687Y]

Protocols for Acetyl Coenzyme A Carboxylase antibody [EP687Y]

Price and availability of products related to Acetyl Coenzyme A Carboxylase antibody [EP687Y]

 

Acetyl Coenzyme A Carboxylase antibody [EP687Y] - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for Acetyl Coenzyme A Carboxylase antibody [EP687Y]

ab30133

  

A431 (Human epithelial carcinoma cell line) Cytoplasmic Lysate - tumor cell line

ab4348

  

Liver (human) normal tissue slides

 

Compatible Secondaries for Acetyl Coenzyme A Carboxylase antibody [EP687Y]

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other Acetyl Coenzyme A Carboxylase Antibodies

See all Acetyl Coenzyme A Carboxylase antibodies (6)...

 

Acetyl Coenzyme A Carboxylase antibody [EP687Y] - other tools:

Contact Abcam with a Technical Enquiry about Acetyl Coenzyme A Carboxylase antibody [EP687Y]

 

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