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I have used this antibody in western blotting and get no bands; at 1:500; in mouse liver lysate, expresses detectable levels of target, other antibodies work well with same sample |
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ANSWER: |
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab45174. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
This image shows human colon carcinoma tissue stained with ab63485 at 1/50 dilution; with and without the immunising peptide.
All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody (ab63485) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract (5-30µg total protein) treated with PMA (125ng/ml, 15min).
Lane 2 : NIH/3T3 cell extract (5-30µg total protein) treated with PMA (125ng/ml, 15min) and the immunising peptide (5-10µg).
Predicted band size : 266 kDa
ICC/IF image of ab63485 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63485, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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