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Read our guarantee »Products:Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
Anti-Acetyl Coenzyme A Carboxylase antibody
See all Acetyl Coenzyme A Carboxylase products (7) ...
Rabbit polyclonal to Acetyl Coenzyme A Carboxylase
WB, IHC-P, ELISA, ICC/IFmore details
Reacts with
Mouse, Rat, Human
Synthetic peptide derived from human Acetyl Coenzyme A Carboxylase around the phosphorylation site of serine 80, (S-M-SP-G-L).
IHC-P: Human colon carcinoma tissue WB: NIH/3T3 cell extract treated with PMA (125ng/ml, 15min).
Liquid
Store at -20°C. Stable for 12 months at -20°C
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Acetyl Coenzyme A Carboxylase antibody (ab63485)
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Western blot - Acetyl Coenzyme A Carboxylase antibody (ab63485)
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Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody (ab63485)
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Our Abpromise guarantee covers the use of ab63485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 266 kDa.
IHC-P: 1/50 - 1/100.
ELISA: 1/5000
ICC/IF: Use a concentration of 5 µg/ml
In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl CoA. In fatty acid synthesis, catalytic formation of malonyl CoA (precursor for long chain fatty acyl CoA, LCFA CoA) from acetyl CoA by Acetyl CoA carboxylase (ACC1) is the rate limiting step. The translocation of LCFA CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT1 and CPT2) and regulated by ACC2, the rate limiting step of mitochondrial fatty acid beta oxidation. Activities of ACC1 and 2 are regulated by their phosphorylation by 5' AMP activated protein kinase (AMPK). Diabetes deranges AMPK master switch and represses the ACC1 gene expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC1 is also known as ACC alpha is a cytosolic enzyme, enriched in liver, adipose and lactating mammary tissues. ACC1 catalyzes the carboxylation of acetyl CoA to form malonyl CoA, the rate limiting step in the biogenesis of LCFA CoA. ACC1 carries three functions: biotin carboxyl carrier protein, biotin carboxylase, and carboxyltransferase (catalytic activity). Two variants of ACC1 have been described. One with 8 additional amino acids commencing at Pro 1196. The other which is 59aa shorter than the predominant fat and liver isoform exist in mammals. The presence of 8 additional amino acids inhibits the in vitro phosphorylation of the Ser1200 by cAMP dependent kinase. The two ACC1 isoform are differentially regulated in a tissue specific manner and under different physiological conditions. The activity of ACC1 is finely regulated by hormone dependent phosphorylation and dephosphorylation.
Cytoplasmic
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Acetyl Coenzyme A Carboxylase antibody (ab63485)

This image shows human colon carcinoma tissue stained with ab63485 at 1/50 dilution; with and without the immunising peptide.
Western blot - Acetyl Coenzyme A Carboxylase antibody (ab63485)

All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody (ab63485) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract (5-30µg total protein) treated with PMA (125ng/ml, 15min).
Lane 2 : NIH/3T3 cell extract (5-30µg total protein) treated with PMA (125ng/ml, 15min) and the immunising peptide (5-10µg).
Predicted band size : 266 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody (ab63485)

ICC/IF image of ab63485 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63485, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab63485 has not yet been referenced specifically in any publications.
Publishing research using ab63485? Please let us know so that we can cite the reference in this datasheet
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This image shows human colon carcinoma tissue stained with ab63485 at 1/50 dilution; with and without the immunising peptide.

All lanes : Anti-Acetyl Coenzyme A Carboxylase antibody (ab63485) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract (5-30µg total protein) treated with PMA (125ng/ml, 15min).
Lane 2 : NIH/3T3 cell extract (5-30µg total protein) treated with PMA (125ng/ml, 15min) and the immunising peptide (5-10µg).
Predicted band size : 266 kDa

ICC/IF image of ab63485 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63485, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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