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Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540)


  • Product nameAnti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y]
    See all Acetyl Coenzyme A Carboxylase primary antibodies
  • Description
    Rabbit monoclonal [EPR3143Y] to Acetyl Coenzyme A Carboxylase (phospho S221)
  • Tested applicationsWB, IP, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues surrounding serine 221 of Human Acetyl Coenzyme A Carboxylase.

  • Positive control
    • HeLa cell lysate treated with calyculin A; Human muscles.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Our Abpromise guarantee covers the use of ab109540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 277 kDa.
IP 1/10.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionCatalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
    • Tissue specificityExpressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
    • PathwayLipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
    • Involvement in diseaseAcetyl-CoA carboxylase 1 deficiency
    • Sequence similaritiesContains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • Post-translational
      Phosphorylation on Ser-1263 is required for interaction with BRCA1.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAC antibody
      • ACACA antibody
      • ACACA_HUMAN antibody
      • ACACB antibody
      • ACC alpha antibody
      • ACC antibody
      • ACC beta antibody
      • ACC-alpha antibody
      • ACC1 antibody
      • ACC2 antibody
      • ACCA antibody
      • ACCB antibody
      • Acetyl CoA carboxylase 1 antibody
      • Acetyl CoA carboxylase 2 antibody
      • Acetyl CoA carboxylase alpha antibody
      • Acetyl CoA carboxylase beta antibody
      • Acetyl Coenzyme A carboxylase alpha antibody
      • Acetyl Coenzyme A carboxylase beta antibody
      • Biotin carboxylase antibody
      • COA1 antibody
      • COA2 antibody
      • HACC275 antibody
      • OTTHUMP00000164069 antibody
      • OTTHUMP00000164070 antibody
      • OTTHUMP00000164076 antibody
      • OTTHUMP00000240532 antibody
      see all

    Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] images

    • All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540) at 1/5000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 277 kDa
    • ab109540, at 1/100 dilution, staining paraffin-embedded Human muscles by Immunohistochemistry.

    References for Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540)

    ab109540 has not yet been referenced specifically in any publications.

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