RabMAb

Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540)

Overview

  • Product nameAnti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y]
    See all Acetyl Coenzyme A Carboxylase primary antibodies
  • Description
    Rabbit monoclonal [EPR3143Y] to Acetyl Coenzyme A Carboxylase (phospho S221)
  • Tested applicationsWB, IP, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues surrounding serine 221 of Human Acetyl Coenzyme A Carboxylase.

  • Positive control
    • HeLa cell lysate treated with calyculin A; Human muscles.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Applications

Our Abpromise guarantee covers the use of ab109540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 277 kDa.
IP 1/10.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • RelevanceIn cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl CoA. In fatty acid synthesis, catalytic formation of malonyl CoA (precursor for long chain fatty acyl CoA, LCFA CoA) from acetyl CoA by Acetyl CoA carboxylase (ACC1) is the rate limiting step. The translocation of LCFA CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT1 and CPT2) and regulated by ACC2, the rate limiting step of mitochondrial fatty acid beta oxidation. Activities of ACC1 and 2 are regulated by their phosphorylation by 5' AMP activated protein kinase (AMPK). Diabetes deranges AMPK master switch and represses the ACC1 gene expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC1 is also known as ACC alpha is a cytosolic enzyme, enriched in liver, adipose and lactating mammary tissues. ACC1 catalyzes the carboxylation of acetyl CoA to form malonyl CoA, the rate limiting step in the biogenesis of LCFA CoA. ACC1 carries three functions: biotin carboxyl carrier protein, biotin carboxylase, and carboxyltransferase (catalytic activity). Two variants of ACC1 have been described. One with 8 additional amino acids commencing at Pro 1196. The other which is 59aa shorter than the predominant fat and liver isoform exist in mammals. The presence of 8 additional amino acids inhibits the in vitro phosphorylation of the Ser1200 by cAMP dependent kinase. The two ACC1 isoform are differentially regulated in a tissue specific manner and under different physiological conditions. The activity of ACC1 is finely regulated by hormone dependent phosphorylation and dephosphorylation.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • ACAC antibody
      • ACACB antibody
      • ACC alpha antibody
      • ACC antibody
      • ACC beta antibody
      • ACC1 antibody
      • ACC2 antibody
      • ACCA antibody
      • ACCB antibody
      • Acetyl CoA carboxylase 1 antibody
      • Acetyl CoA carboxylase 2 antibody
      • Acetyl CoA carboxylase alpha antibody
      • Acetyl CoA carboxylase beta antibody
      • Acetyl Coenzyme A carboxylase alpha antibody
      • Acetyl Coenzyme A carboxylase beta antibody
      • COA1 antibody
      • COA2 antibody
      • HACC275 antibody
      • OTTHUMP00000164069 antibody
      • OTTHUMP00000164070 antibody
      • OTTHUMP00000164076 antibody
      • OTTHUMP00000240532 antibody
      see all

    Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] images

    • All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540) at 1/5000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 277 kDa
    • ab109540, at 1/100 dilution, staining paraffin-embedded Human muscles by Immunohistochemistry.

    References for Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540)

    ab109540 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"