Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540)

Overview

• Product nameAnti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y]See all Acetyl Coenzyme A Carboxylase primary antibodies ...
• Description
  Rabbit monoclonal [EPR3143Y] to Acetyl Coenzyme A Carboxylase (phospho S221)
• Tested applicationsWB, IP, IHC-P, ICC more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  A synthetic peptide corresponding to residues surrounding serine 221 of Human Acetyl Coenzyme A Carboxylase.

• Positive controlHeLa cell lysate treated with calyculin A; Human muscles.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR3143Y
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Fatty acid oxidation

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Fatty Acids
  • Synthesis

Applications

Target

• RelevanceIn cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl CoA. In fatty acid synthesis, catalytic formation of malonyl CoA (precursor for long chain fatty acyl CoA, LCFA CoA) from acetyl CoA by Acetyl CoA carboxylase (ACC1) is the rate limiting step. The translocation of LCFA CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT1 and CPT2) and regulated by ACC2, the rate limiting step of mitochondrial fatty acid beta oxidation. Activities of ACC1 and 2 are regulated by their phosphorylation by 5' AMP activated protein kinase (AMPK). Diabetes deranges AMPK master switch and represses the ACC1 gene expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC1 is also known as ACC alpha is a cytosolic enzyme, enriched in liver, adipose and lactating mammary tissues. ACC1 catalyzes the carboxylation of acetyl CoA to form malonyl CoA, the rate limiting step in the biogenesis of LCFA CoA. ACC1 carries three functions: biotin carboxyl carrier protein, biotin carboxylase, and carboxyltransferase (catalytic activity). Two variants of ACC1 have been described. One with 8 additional amino acids commencing at Pro 1196. The other which is 59aa shorter than the predominant fat and liver isoform exist in mammals. The presence of 8 additional amino acids inhibits the in vitro phosphorylation of the Ser1200 by cAMP dependent kinase. The two ACC1 isoform are differentially regulated in a tissue specific manner and under different physiological conditions. The activity of ACC1 is finely regulated by hormone dependent phosphorylation and dephosphorylation.
• Cellular localizationCytoplasmic
• Database links
  • Entrez Gene: 31 Human
  • Entrez Gene: 32 Human
  • Entrez Gene: 32 Human
  • Omim: 200350 Human
  • Omim: 601557 Human
  • SwissProt: Q13085 Human
  • SwissProt: O00763 Human
  • SwissProt: O00763 Human

  • Unigene: 160556 Human
  • Unigene: 234898 Human

  see all

• Alternative names
  ACAC antibodyACACB antibodyACC alpha antibody

  ACC antibodyACC beta antibodyACC1 antibodyACC2 antibodyACCA antibodyACCB antibodyAcetyl CoA carboxylase 1 antibodyAcetyl CoA carboxylase 2 antibodyAcetyl CoA carboxylase alpha antibodyAcetyl CoA carboxylase beta antibodyAcetyl Coenzyme A carboxylase alpha antibodyAcetyl Coenzyme A carboxylase beta antibodyCOA1 antibodyCOA2 antibodyHACC275 antibodyOTTHUMP00000164069 antibodyOTTHUMP00000164070 antibodyOTTHUMP00000164076 antibodyOTTHUMP00000240532 antibody

  see all

Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] images

• 

  Western blot - Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540)
  All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540) at 1/5000 dilution
  
  Lane 1 : HeLa cell lysate, untreated
  Lane 2 : HeLa cell lysate treated with calyculin A
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP-labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 277 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540)
  ab109540, at 1/100 dilution, staining paraffin-embedded Human muscles by Immunohistochemistry.