Products:Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
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All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S80) antibody (ab55177) at 1/500 dilution
Lane 1 : extracts from 293 cells treated with
EGF (200ng/ml, 5mins) and treated with the immunising phosphopeptide.
Lane 2 : extracts from 293 cells treated with
EGF (200ng/ml, 5mins).
Predicted band size : 265 kDa
Observed band size : >175 kDa (why is the actual band size different from the predicted?)
Immunohistochemical analysis of Acetyl Coenzyme A Carboxylase (phospho S80) expression in paraffin embedded human breast carcinoma tissue using 1/50 ab55177. Left: no phosphopeptide. Right: sample incubated with immunising phosphopeptide.
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