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Products:Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
Anti-Acetyl Coenzyme A carboxylase alpha antibody [EP687Y] (ab78274)
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- Secondary antibodies
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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - acetyl Coenzyme A carboxylase alpha antibody (ab78274)
Anti-Acetyl Coenzyme A carboxylase alpha antibody [EP687Y] (ab78274) at 1/2000 dilution + A431 cell lysate
Secondary
Goat anti-rabbit HRP conjugate at 1/5000 dilution
developed using the ECL technique
Predicted band size : 266 kDa
Observed band size : 266 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - acetyl Coenzyme A carboxylase alpha antibody (ab78274)
ab78274, at a 1/50 dilution, staining acetyl Coenzyme A carboxylase alpha in paraffin embedded human liver tissue by Immunohistochemistry.
](/ps/datasheet/images/78/ab78274/Acetyl-Coenzyme-A-carboxylase-alpha-Primary-antibodies-ab78274-1.jpg)
Flow Cytometry-Anti-Acetyl Coenzyme A carboxylase alpha antibody [EP687Y](ab78274)
Overlay histogram showing HEK293 cells stained with ab78274 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78274, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
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