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Products:Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
Overview
Product name
Acetyl Coenzyme A carboxylase alpha protein (His tag)
Protein description
Recombinant fragment corresponding to amino acids 39-2346 of Human Acetyl Coenzyme A carboxylase alpha with C terminal His tag expressed in a Baculovirus infected Sf9 cell expression system, 266.5kDa. Swiss ID = Q13085
Properties
Purification notes
Purity: >50% by SDS-PAGE.
Form
Liquid
Storage instructions
Store at -20°C, Stable for 6 months at -20°C
Storage buffer
Preservative: None
Constituents: 10% Glycerol, 0.05% Tween 20, 50mM Tris HCl, 3mM DTT, 150mM Sodium chloride, 0.1mM EDTA, 0.1mM EGTA, pH 7.5
Concentration
Concentration information loading...
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE
Application notes
SDS-PAGE: Use at an assay dependent dilution.
ab79625 is useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
Tissue specificity
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
Pathway
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
Involvement in disease
Defects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:200350]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain.
Post-translational
modifications
Phosphorylation on Ser-1263 is required for interaction with BRCA1.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ13085
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for Acetyl Coenzyme A carboxylase alpha protein (His tag) (ab79625)
ab79625 has not yet been referenced specifically in any publications.
Publishing research using ab79625? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"