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Acetylcholine (conjugated) antibody [HYChol001] (ab37010)

Submit an Abreview Q&A (6)Specific References (1)

Overview

  • Product nameAcetylcholine (conjugated) antibody [HYChol001]See all Acetylcholine primary antibodies ...
  • Description
    Mouse monoclonal [HYChol001] to Acetylcholine (conjugated)
  • SpecificityThe antibody specifically recognizes choline and acetylcholine bound by glutaric anhydride to a protein carrier. Free acetylcholine is too small to be recognized by immunoglobulin. This antibody is a tool to visualise acetylcholine bound to biological structure such as receptors. This antibody is specific for conjugated Choline-glutaric acid. Cross reactivity (concentration at half displacement) : Choline-Glutaric acid-Protein carrier : 1 Choline-Adipic acid-Protein carrier : 1/500 Choline-Succinic acid-Protein carrier : 1/2500 Farnesol-Protein carrier : 1/>50000 Retinoic acid-Protein carrier : 1/>50000 Myristic acid-Protein carrier : 1/>50000 Acetylcholine : 1/>50000 Choline : 1/>50000
  • Tested applicationsWB, ELISA, ICC, IHC-Fr more details
  • Immunogen

    Chemical / Small Molecule, Choline-Glutaric acid conjugated to protein carrier.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, ddH20
  • Concentration information loading...
  • PurityAscites
  • Purification notesThis antibody was purified by ammonium sulfate precipitation.
  • Clonality Monoclonal
  • Clone numberHYChol001
  • MyelomaSp2/0-Ag14
  • IsotypeIgG2a
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab37010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/2000.
ELISA ELISA: 1/1000 - 1/5000.
ICC ICC: 1/1000 - 1/5000.
IHC-Fr IHC-Fr: 1/1000 - 1/2000.

Target

  • RelevanceThe chemical compound Acetylcholine, often abbreviated as ACh, was the first neurotransmitter to be identified. It is a chemical transmitter in the central nervous system (CNS) as well as in the parasympathetic nervous system in many organisms including humans. When it binds to Acetylcholine receptors of muscle fibers, stimulates those fibers to contract. Acetylcholine is also used in the brain, where it tends to cause excitatory actions.
  • Cellular localizationCytoplasmic and Secreted
  • Alternative names
      [2 (acetyloxy)ethyl]trimethylammonium antibodyACh antibody

References for Acetylcholine (conjugated) antibody [HYChol001] (ab37010)

This product has been referenced in:
  • de Moura Júnior NB  et al. Expression of acetylcholine and its receptor in human sympathetic ganglia in primary hyperhidrosis. Ann Thorac Surg 95:465-70 (2013). IHC ; Human . Read more (PubMed: 23219256) »

See 1 Publication for this product

Publishing research using ab37010 ? Please let us know so that we can cite the reference in this datasheet

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Submit an Abreview for ab37010 Submit a Question for ab37010

Displaying 1 - 6 of 6 results for Abreviews and Q&A

10
Votes: Highest First

Does this work both in human and mouse tissue?

This antibody has been tested in Rat.

Thank you for your quick answer, I am asking this to be able to know if i have to buy 1 or 2, they are small bowel biopsies size no more than one centimeter.

For the dilutions that my colleague mentioned in his e-mail, one unit (that is, one vial) will stain 100 - 500 sections. Each section should require not more than 100 microliters, and if the biopsies are 1 centimeter square, even 50 microliters should be ...

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Can you please explain me how many test can I get of this antibodies using IHC-FR (INMUNOHISTOCHEMISTRY) technique?



cat # AB37010 Acetylcholine (conjugated) antibody [HYChol001] 50 µl

cat # AB3523 Anti-iNOS ant...

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Thank you for your enquiry. Here are answers to your questions:

Using AB37010 at the recommended starting dilution of 1:1000 will produce a volume of 50ml working solution.

Using AB3523at the recommended starting dilution of 1:50 will ...

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Thank you, I also wanted to ask about Anti-Acetylcholine antibody (ab34935). Would this work in the same way? ie only detecting bound acetylcholine? I was under the impression that polyclonal antibodies were generated against KLH conjugated to ACh meaning...

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Thank you for your inquiry.

You are correct that the immunogen would be Acetylcholine coupled to some carrier. When polyclonal antibodies are generated some will be specific for Acetylcholine.

The antibodies that are specific for the c...

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Product code: 37010

Inquiry: I am somewhat confused as to the function of this Ab (37010). I understand that this antibody recognizes acetylcholine bound by glutaric anhydride to a protein carrier. Does this mean that we must conjugate ACh and ...

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Thank you for your inquiry.

I can confirm that free acetylcholine is too small to be recognized by antibodies.

ab37010 will recognize only bound acetylcholine. Acetylcholine can be bound to a receptor for example and then the antibody ...

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BATCH NUMBER 318966 ORDER NUMBER 259212 DESCRIPTION OF THE PROBLEM Non-specific staining, everything was dim SAMPLE neuron cell culture from Xenopus Laevis PRIMARY ANTIBODY ab37010, 1:1000 or 1:200 (no difference between the two...

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Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab37010. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a produc...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"