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Acid Phosphatase Assay Kit (Colorimetric) (ab83367)

Q&A (1)

Overview

  • Product nameAcid Phosphatase Assay Kit (Colorimetric)See all Acid phosphatase kits ...
  • Tests
    500 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeEnzyme activity
  • Sensitivity
    > 0.001 mM
  • Range
    0.001 mM - 1 mM
  • Product overview

    Abcam's Acid Phosphatase Assay Kit (Colorimetric) is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (?max = 405 nm) when dephosphorylated by AP.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceAcid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.
  • Cellular localizationACP1: Cytoplasm. ACP2: Lysosome membrane; Single-pass membrane protein. ACP5: Lysosome. ACPP: Isoform 1: Secreted. Isoform 2: Lysosome membrane; Single-pass type I membrane protein.
    • Alternative names
        Acid phosphatase 1 solubleAcid phosphatase 2 lysosomalAcid phosphatase 5 tartrate resistant
        Acid phosphatase of erythrocyteAcid phosphatase prostateACP1ACP2ACP3ACP5ACPPAdipocyte acid phosphataseHAAPLAPLMW PTPLMW PTPaseLow molecular weight cytosolic acid phosphataseLow molecular weight phosphotyrosine protein phosphataseLysosomal acid phosphatasePAPProstatic acid phosphotaseRed cell acid phosphatase 1Tartrate resistant acid ATPaseTartrate resistant acid phosphatase 5Tartrate resistant acid phosphatase type 5TR APTRAPTrATPase
      see all

    Applications

    Our Abpromise guarantee covers the use of ab83367 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Acid Phosphatase Assay Kit (Colorimetric) images

    • Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
      Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
      p-Nitrophenol (pNP) standard curve
    • Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
      Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
      AP Enzyme samples 40 minute assay

    Protocols

    References for Acid Phosphatase Assay Kit (Colorimetric) (ab83367)

    ab83367 has not yet been referenced specifically in any publications.

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    Could you please provide the buffer constituents details with information on molar, pH, presence/absence of protease inhibitor, etc?

    Thank you for your enquiry.

    Unfortunately, most of the information you asked for is proprietary info. However, I can confirm that the assay buffer does not contain protease inhibitors and its pH is 5.0.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"