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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
Overview
Product name
Acid Phosphatase Colorimetric Assay Kit
See all Acid phosphatase products (2) ...
Product overview
Acid Phosphatase Colorimetric Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure Acid Phosphatase (AP) activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (Lambda max = 405 nm) when dephosphorylated by AP. The kit can detect µU acid phosphatase activity in samples.
Notes
Protect kit from light. Briefly centrifuge vials before opening.
pNPP Tablets: NEVER TOUCH THE TABLETS WITH BARE HANDS.
AP Enzyme Solution: NEVER FREEZE! The enzymes are stable for up to 2 months at 4oC after reconstitution.
Allow Assay Buffer to warm to room temperature before use. Keep pNNP substrate solution, and AP Enzyme on ice during the assay.
pNPP Tablets: NEVER TOUCH THE TABLETS WITH BARE HANDS.
AP Enzyme Solution: NEVER FREEZE! The enzymes are stable for up to 2 months at 4oC after reconstitution.
Allow Assay Buffer to warm to room temperature before use. Keep pNNP substrate solution, and AP Enzyme on ice during the assay.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
| Components | Identifier | 500 tests |
|---|---|---|
| AP Assay buffer | NM | 1 x 100ml |
| AP Enzyme (Lyophilised) | Green | 1 vial |
| pNPP Substrate (10 tablets) | Red | 1 vial |
| Stop Solution | WM | 1 x 10ml |
Research areas
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
Cancer >> Tumor biomarkers >> Enzymes >> Phosphatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
Relevance
Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.
Cellular localization
ACP1: Cytoplasm. ACP2: Lysosome membrane; Single-pass membrane protein. ACP5: Lysosome. ACPP: Isoform 1: Secreted. Isoform 2: Lysosome membrane; Single-pass type I membrane protein.
Alternative names
- Acid phosphatase 1 soluble
- Acid phosphatase 2 lysosomal
- Acid phosphatase 5 tartrate resistant
see all
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use at an assay dependent dilution.
Acid Phosphatase Colorimetric Assay Kit images:
Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
p-Nitrophenol (pNP) standard curve
Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
AP Enzyme samples 40 minute assay
References for Acid Phosphatase Colorimetric Assay Kit (ab83367)
ab83367 has not yet been referenced specifically in any publications.
Publishing research using ab83367? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"