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Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
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Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
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Product Name
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Acid Phosphatase Colorimetric Assay Kit
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Product type
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Kits
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Description
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Acid Phosphatase Colorimetric Assay Kit
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Product overview
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Acid Phosphatase Colorimetric Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure Acid Phosphatase (AP) activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (Lambda max = 405 nm) when dephosphorylated by AP. The kit can detect µU acid phosphatase activity in samples.
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Components
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| Kit Components | Unit Size/Volume | Cap Colour |
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| AP Assay buffer | 100ml | NM | | pNPP Substrate (10 tablets) | 1 vial | Red | | AP Enzyme | 1 vial | Green | | Stop Solution | 10ml | WM |
Note: Please Click Here for Instructions For Use/Protocol
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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Tested applications
(see key)
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FuncS
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Notes
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Protect kit from light. Briefly centrifuge vials before opening.
pNPP Tablets: NEVER TOUCH THE TABLETS WITH BARE HANDS.
AP Enzyme Solution: NEVER FREEZE! The enzymes are stable for up to 2 months at 4oC after reconstitution.
Allow Assay Buffer to warm to room temperature before use. Keep pNNP substrate solution, and AP Enzyme on ice during the assay.
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Material safety datasheet (MSDS) for this product:
Acid Phosphatase Colorimetric Assay Kit MSDS
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Relevance
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Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Acid Phosphatase Colorimetric Assay Kit is on this datasheet. But please do contact us if you would like any reassurance! |
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Acid Phosphatase Colorimetric Assay Kit images:
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 Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
p-Nitrophenol (pNP) standard curve
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Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
AP Enzyme samples 40 minute assay
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Search PubMed (MEDLINE) for references to Acid phosphatase
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Acid Phosphatase Colorimetric Assay Kit - more information
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Customer reviews (feedback) regarding Acid Phosphatase Colorimetric Assay Kit |
Customer FAQs regarding Acid Phosphatase Colorimetric Assay Kit |
Protocols for Acid Phosphatase Colorimetric Assay Kit |
Price and availability of products related to Acid Phosphatase Colorimetric Assay Kit |
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Acid Phosphatase Colorimetric Assay Kit - other tools:
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Contact Abcam with a Technical Enquiry about Acid Phosphatase Colorimetric Assay Kit |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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