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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
Overview
Product name
Acid Phosphatase Fluorometric Assay Kit
See all Acid phosphatase products (2) ...
Product overview
Acid Phosphatase Fluorometric Assay Kit uses non-fluorescent Methylumbelliferyl phosphate disodium (MUP) as the substrate which has Ex/Em=360/440 nm when dephosphorylated by AP. The kit is an ultra-sensitive, simple, direct and HTS-ready fluorometric assay designed to measure AP activity in serum and other biosamples. The detection sensitivity is ~1 µU, more sensitive than colorimetric assays. The kit is suitable for both research and drug discovery.
Notes
Protect kit from light. Briefly centrifuge vials before opening.
MUP: The reconstituted MUP solution is stable for 2 months at -20°C.
AP Enzyme: The enzyme is stable for up to 2 months at 4°C after reconstitution. DO NOT FREEZE!
Ensure that the Assay Buffer is at room temperature before use. Keep samples and AP Enzyme on ice during the assay.
MUP: The reconstituted MUP solution is stable for 2 months at -20°C.
AP Enzyme: The enzyme is stable for up to 2 months at 4°C after reconstitution. DO NOT FREEZE!
Ensure that the Assay Buffer is at room temperature before use. Keep samples and AP Enzyme on ice during the assay.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
| Components | Identifier | 500 tests |
|---|---|---|
| AP Assay buffer | NM | 1 x 100ml |
| AP Enzyme (Lyophilised) | Green | 1 vial |
| MUP Substrate | Red | 1 vial |
| Stop Solution | WM | 100 x 250ml |
Research areas
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
Cancer >> Tumor biomarkers >> Enzymes >> Phosphatases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
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Functional Studies - Acid Phosphatase Fluorometric Assay Kit (ab83370)
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Relevance
Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.
Cellular localization
ACP1: Cytoplasm. ACP2: Lysosome membrane; Single-pass membrane protein. ACP5: Lysosome. ACPP: Isoform 1: Secreted. Isoform 2: Lysosome membrane; Single-pass type I membrane protein.
Alternative names
- Acid phosphatase 1 soluble
- Acid phosphatase 2 lysosomal
- Acid phosphatase 5 tartrate resistant
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab83370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use at an assay dependent dilution.
Acid Phosphatase Fluorometric Assay Kit images:
Functional Studies - Acid Phosphatase Fluorometric Assay Kit (ab83370)
4-Methylumbelliferone (4-MU) Standard Curve.
References for Acid Phosphatase Fluorometric Assay Kit (ab83370)
ab83370 has not yet been referenced specifically in any publications.
Publishing research using ab83370? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"