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Acid Phosphatase protein (Active) (ab87493)

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Overview

  • Product nameAcid Phosphatase protein (Active)See all Acid Phosphatase proteins and peptides ...
  • Protein descriptionRecombinant full length Human Acid Phosphatase (amino acids 1-158) fused to a His tag at the N-terminus; 178 amino acids, 20.1kDa (Swiss-Prot P24666).
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesab87493 is purified using conventional chromatography techniques.
  • Biological activitySpecific activity: > 60,000 unit/mg of protein. Unit definition: One unit is defined as the amount of enzyme that will hydrolyze 1nmole of p-nitrophenyl phosphate per minute at 37oC in MES pH5.0 using 10mM of substrate.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Glycerol, 20mM MES, 2mM EDTA, 0.1mM PMSF, pH 6.0
  • Concentration information loading...
  • Additional notesEndotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).
  • Sequence notesMGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab87493 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab166896.

    • Protein info

    • Alternative names
        Acid phosphatase 1 solubleAcid phosphatase of erythrocyteACP1
        Adipocyte acid phosphataseCytoplasmic phosphotyrosyl protein phosphataseHAAPLMW-PTPLMW-PTPaseLow molecular weight cytosolic acid phosphataseLow molecular weight phosphotyrosine protein phosphatasePAP1PAP2PPAC_HUMANProtein tyrosine phosphatasePTPasePurple acid phosphataseRed cell acid phosphatase 1
      see all
  • FunctionActs on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
  • Tissue specificityT-lymphocytes express only isoform 2.
  • Sequence similaritiesBelongs to the low molecular weight phosphotyrosine protein phosphatase family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P24666 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Acid Phosphatase protein (Active) images

    • SDS-PAGE - Acid Phosphatase protein (Active) (ab87493)
      SDS-PAGE - Acid Phosphatase protein (Active) (ab87493)
      15% SDS-PAGE showing 3µg ab87493 at approximately 20.1kDa.

    References for Acid Phosphatase protein (Active) (ab87493)

    ab87493 has not yet been referenced specifically in any publications.

    Publishing research using ab87493 ? Please let us know so that we can cite the reference in this datasheet

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