You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Acid Phosphatase protein (Tagged-His Tag) (ab91862)

Submit an Abreview Submit a Question

Overview

  • Product nameAcid Phosphatase protein (Tagged-His Tag)See all Acid Phosphatase proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 4-158 (155 aa) of Human Acid Phosphatase with a N terminal His tag; 21 kDa (predicted MWt 19kDa). Uniprot ID = P24666.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution with 114 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesQATKSVLFVCLGNICRSPIAEAVFRKLVTDQNISENWRVD SAATSGYEIGNPPD YRGQSCMKRHGIPMSHVARQITKE DFATFDYILCMDESNLRDLNRKSNQVKTCK AKIELLGS YDPQKQLIIEDPYYGNDSDFETVYQQCVRCCRAFLEKAH
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91862 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Acid phosphatase 1 solubleAcid phosphatase of erythrocyteACP1
        Adipocyte acid phosphataseCytoplasmic phosphotyrosyl protein phosphataseHAAPLMW-PTPLMW-PTPaseLow molecular weight cytosolic acid phosphataseLow molecular weight phosphotyrosine protein phosphatasePAP1PAP2PPAC_HUMANProtein tyrosine phosphatasePTPasePurple acid phosphataseRed cell acid phosphatase 1
      see all
  • FunctionActs on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
  • Tissue specificityT-lymphocytes express only isoform 2.
  • Sequence similaritiesBelongs to the low molecular weight phosphotyrosine protein phosphatase family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P24666 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Acid Phosphatase protein (Tagged-His Tag) images

    • SDS-PAGE - Acid Phosphatase protein (Tagged-His Tag) (ab91862)
      SDS-PAGE - Acid Phosphatase protein (Tagged-His Tag) (ab91862)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Acid Phosphatase protein (Tagged-His Tag) (ab91862)

    ab91862 has not yet been referenced specifically in any publications.

    Publishing research using ab91862 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab91862 Submit a Question for ab91862

    There are currently no Abreviews or Questions for ab91862.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"