Anti-Calponin 3 antibody - C-terminal (ab204365)
Key features and details
- Rabbit polyclonal to Calponin 3 - C-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calponin 3 antibody - C-terminal
See all Calponin 3 primary antibodies -
Description
Rabbit polyclonal to Calponin 3 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human Calponin 3 aa 250 to the C-terminus (C terminal).
Database link: Q15417 -
Positive control
- WB: RT-4 & U-251 MG whole cell lysates; IHC-P: Human placenta, kidney and testis tissue. ICC: U-2 OS whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 36 kDa.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 36 kDa. |
Target
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Function
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. -
Tissue specificity
Expressed in both non-smooth muscle tissues as well as smooth muscle tissues. -
Sequence similarities
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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Database links
- Entrez Gene: 538975 Cow
- Entrez Gene: 1266 Human
- Entrez Gene: 71994 Mouse
- Entrez Gene: 54321 Rat
- Omim: 602374 Human
- SwissProt: Q32L92 Cow
- SwissProt: Q15417 Human
- SwissProt: Q9DAW9 Mouse
see all -
Alternative names
- Acidic calponin 3 antibody
- acidic isoform antibody
- Calponin 3 acidic antibody
see all
Images
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All lanes : Anti-Calponin 3 antibody - C-terminal (ab204365) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Predicted band size: 36 kDa -
Immunohistochemical analysis of human placenta tissue labeling Calponin 3 in epithelial cells with ab204365 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling Calponin 3 in the cytosol and actin filaments with ab204365 at 2 µg/mL.
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Immunohistochemical analysis of human kidney tissue labeling Calponin 3 in membrane and cytoplasm of cells in tubules with ab204365 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human testis tissue labeling Calponin 3 in peritubular myoid cells with ab204365 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human skeletal muscle tissue labeling Calponin 3 in myocytes with ab204365 at a 1/1000 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab204365 has been referenced in 1 publication.
- Indra I et al. Sensing Actin Dynamics through Adherens Junctions. Cell Rep 30:2820-2833.e3 (2020). PubMed: 32101754