Anti-Acidic Cytokeratin antibody [AE-1] (ab187381)
Key features and details
- Mouse monoclonal [AE-1] to Acidic Cytokeratin
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Acidic Cytokeratin antibody [AE-1] -
Description
Mouse monoclonal [AE-1] to Acidic Cytokeratin -
Host species
Mouse -
Specificity
ab187381 recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Acidic Cytokeratin. P13645 (CK10); P02533 (CK14); P19012 (CK15); P08789 (CK16); P08727 (CK19)
Database link: P13645 -
Positive control
- Skin, Squamous cell carcinoma (SCC)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AE-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 66 kDa.
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Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 66 kDa. |
Target
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Relevance
Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3858 Human
- Omim: 148080 Human
- SwissProt: P13645 Human
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Alternative names
- CK10 antibody
- Cytokeratin 10 antibody
- K10 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab187381 has not yet been referenced specifically in any publications.