Anti-Acidic Cytokeratin antibody [AE1] (ab3117)

Overview

• Product nameAnti-Acidic Cytokeratin antibody [AE1]See all Acidic Cytokeratin primary antibodies ...
• Description
  Mouse monoclonal [AE1] to Acidic Cytokeratin
• SpecificityThis antibody reacts with MCF-7, RT 112 and A 431 cultured cell lines.
• Tested applicationsICC, IHC-P, IHC-Fr, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Human, Monkey
  Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow
• Immunogen

  Human epidermal keratin.

• Positive controlSkin. Squamous cellcarcinoma.
• General notes

  
  

  Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. The basic keratins have molecular weights of 65 - 67, 64, 59, 58, 56 and 52kDa. Members of acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with cell type, differentiation status and environment. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
• Concentration information loading...
• PurityProtein G purified
• Primary antibody notes Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. The basic keratins have molecular weights of 65 - 67, 64, 59, 58, 56 and 52kDa. Members of acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with cell type, differentiation status and environment. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
• Clonality Monoclonal
• Clone numberAE1
• IsotypeIgG1
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Intermediate Filaments
  • Class I
  • Cytokeratins

Applications

Target

• RelevanceCytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin.
• Cellular localizationCytoplasmic
• Database links
  • Entrez Gene: 771977 Chicken
  • Entrez Gene: 281888 Cow
  • Entrez Gene: 788909 Cow
  • Entrez Gene: 3858 Human
  • Entrez Gene: 16661 Mouse
  • Entrez Gene: 100009029 Rabbit
  • Entrez Gene: 450225 Rat
  • Omim: 148080 Human

  • SwissProt: P06394 Cow
  • SwissProt: P13645 Human
  • SwissProt: P02535 Mouse
  • SwissProt: Q6IFW6 Rat

  see all

• Alternative names
  67 kDa cytokeratin antibodyCK 1 antibodyCK1 antibody

  CK10 antibodyCytokeratin 10 antibodyHair alpha protein antibodyK 1 antibodyK10 antibodyKeratin 1 antibodyKeratin 10 antibodyKeratin type I cytoskeletal 10 antibodyKeratin type II cytoskeletal 1 antibodyKeratin10 antibodyKPP antibodyKRT10 antibody

  see all

Anti-Acidic Cytokeratin antibody [AE1] images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Acidic Cytokeratin antibody [AE1] (ab3117)
  ab3117 (1/100) staining formalin-fixed, paraffin embedded human colon tissue sections. Antigen retrieval performed by digestion with trypsin at 1mg/ml for 10min at 37^oC.
• 

  Immunocytochemistry/ Immunofluorescence-Acidic Cytokeratin antibody [AE1](ab3117)
  ICC/IF image of ab3117 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3117, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Flow Cytometry - Anti-Acidic Cytokeratin antibody [AE1] (ab3117)
  Overlay histogram showing A431 cells stained with ab3117 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3117, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.