Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cardiovascular >> Blood >> Other
Overview
Product name
Anti-Aconitase 1 antibody
See all Aconitase 1 products (8) ...
Description
Rabbit polyclonal to Aconitase 1
Specificity
ab61197 detects endogenous levels of total Aconitase 1 protein.
Tested applications
ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic non-phosphopeptide derived from human Aconitase 1 around the phosphorylation site of serine 138.
Positive control
HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab61197 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Vitamins / Minerals
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
Cardiovascular >> Blood >> Other
-
Immunocytochemistry/ Immunofluorescence - Aconitase 1 antibody (ab61197)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
ICC/IF: 1/500 - 1/1000.
Target
Function
Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Sequence similarities
Belongs to the aconitase/IPM isomerase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP21399
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACO 1 antibody
- ACO1 antibody
- ACOC_HUMAN antibody
see all
Database links
- Entrez Gene: 48 Human
- Entrez Gene: 11428 Mouse
- Entrez Gene: 50655 Rat
- Omim: 100880 Human
- SwissProt: P21399 Human
- SwissProt: P28271 Mouse
- SwissProt: Q63270 Rat
- Unigene: 567229 Human
see all
Anti-Aconitase 1 antibody images:
Immunocytochemistry/ Immunofluorescence - Aconitase 1 antibody (ab61197)
ab61197 at 1/500 dilution staining Aconitase 1 in HeLa cells by Immunofluorescence, in the absence (left) or presence (right) of the immunising peptide.
References for Anti-Aconitase 1 antibody (ab61197)
ab61197 has not yet been referenced specifically in any publications.
Publishing research using ab61197? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"