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Products:Cardiovascular >> Blood >> Other
Overview
Product name
Anti-Aconitase 1 (phospho S138) antibody
See all Aconitase 1 products (8) ...
Description
Rabbit polyclonal to Aconitase 1 (phospho S138)
Specificity
ab63260 detects endogenous levels of Aconitase 1 only when phosphorylated at serine 138.
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human Aconitase 1 around the phosphorylation site of serine 138 (A-D-SP-L-Q).
Positive control
Human ovary tissue
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab63260 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Vitamins / Minerals
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
Cardiovascular >> Blood >> Other
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Aconitase 1 (phospho S138) antibody (ab63260)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/50 - 1/100.
Target
Function
Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Sequence similarities
Belongs to the aconitase/IPM isomerase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP21399
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACO 1 antibody
- ACO1 antibody
- ACOC_HUMAN antibody
see all
Database links
- Entrez Gene: 48 Human
- Entrez Gene: 11428 Mouse
- Entrez Gene: 50655 Rat
- Omim: 100880 Human
- SwissProt: P21399 Human
- SwissProt: P28271 Mouse
- SwissProt: Q63270 Rat
- Unigene: 567229 Human
see all
Anti-Aconitase 1 (phospho S138) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Aconitase 1 (phospho S138) antibody (ab63260)
Immunohistochemical analysis of Aconitase 1 (phospho S138) expression in paraffin-embedded human ovary tissue using ab63260 at 1/50. Left image: untreated. Right image: sample treated with immunising phosphopeptide.
References for Anti-Aconitase 1 (phospho S138) antibody (ab63260)
ab63260 has not yet been referenced specifically in any publications.
Publishing research using ab63260? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"