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Products:Cardiovascular >> Blood >> Other
Overview
Product name
Anti-Aconitase 1 (phospho S711) antibody
See all Aconitase 1 products (8) ...
Description
Rabbit polyclonal to Aconitase 1 (phospho S711)
Specificity
ab62451 detects endogenous levels of Aconitase 1 only when phosphorylated at serine 711.
Tested applications
IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human Aconitase 1 around the phosphorylation site of serine 711 (Y-G-SP-R-R).
Positive control
Human thyroid gland tissue.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab62451 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Vitamins / Minerals
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Signal Transduction >> Metabolism >> Energy Metabolism
Cardiovascular >> Blood >> Other
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Aconitase 1 (phospho S711) antibody (ab62451)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab62451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Sequence similarities
Belongs to the aconitase/IPM isomerase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP21399
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACO 1 antibody
- ACO1 antibody
- ACOC_HUMAN antibody
see all
Database links
- Entrez Gene: 48 Human
- Entrez Gene: 11428 Mouse
- Entrez Gene: 50655 Rat
- Omim: 100880 Human
- SwissProt: P21399 Human
- SwissProt: P28271 Mouse
- SwissProt: Q63270 Rat
- Unigene: 567229 Human
see all
Anti-Aconitase 1 (phospho S711) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Aconitase 1 (phospho S711) antibody (ab62451)
ab62451, at 1/50 dilution, staining Aconitase 1 in paraffin embedded human thyroid gland tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
References for Anti-Aconitase 1 (phospho S711) antibody (ab62451)
ab62451 has not yet been referenced specifically in any publications.
Publishing research using ab62451? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"