![Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6D1BE4] (ab110320) image](#Aconitase-2-Primary-antibodies-ab110320-4.gif)
![Immunoprecipitation - Aconitase 2 antibody [6D1BE4] (ab110320) image](#Aconitase-2-Primary-antibodies-ab110320-2.jpg)
![Flow Cytometry - Aconitase 2 antibody [6D1BE4] (ab110320) image](#Aconitase-2-Primary-antibodies-ab110320-3.jpg)
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Product name
Anti-Aconitase 2 antibody [6D1BE4]
See all Aconitase 2 products (12) ...
Description
Mouse monoclonal [6D1BE4] to Aconitase 2
Tested applications
IP, ICC/IF, In-Cell ELISA, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Borvine heart mitochondria
Positive control
HDFn cells and HeLa cells; Hela, HepG2, Rat and Mouse liver cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
ab110320 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
6D1BE4
Isotype
IgG2a
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 1 µg/ml.
In-Cell ELISA: Use a concentration of 1 µg/ml.
Flow Cyt: Use a concentration of 1 µg/ml.
Target
Function
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Sequence similarities
Belongs to the aconitase/IPM isomerase family.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionQ99798
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- ACO 2 antibody
- ACO2 antibody
see all
Database links
- Entrez Gene: 50 Human
- Entrez Gene: 50 Human
- Entrez Gene: 11429 Mouse
- Entrez Gene: 11429 Mouse
- Entrez Gene: 79250 Rat
- Entrez Gene: 79250 Rat
- Omim: 100850 Human
- SwissProt: Q99798 Human
see all
Anti-Aconitase 2 antibody [6D1BE4] images:
Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)
![Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)](/ps/datasheet/images/110/ab110320/Aconitase-2-Primary-antibodies-ab110320-4.gif)
ab110320 at 1µg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: the target protein locates to the mitochondria.
Immunoprecipitation - Aconitase 2 antibody [6D1BE4] (ab110320)
![Immunoprecipitation - Aconitase 2 antibody [6D1BE4] (ab110320)](/ps/datasheet/images/110/ab110320/Aconitase-2-Primary-antibodies-ab110320-2.jpg)
Detection of ab110321 by colloidal Coomassie blue G staining of Immunprecipitate.
Staining of Aconitase in HeLa(2), HepG2(3), Rat liver(4), and Mouse liver(5) cell lysates immunoprecipitated using ab110320.
Molecular weight standard (1).
Flow Cytometry - Aconitase 2 antibody [6D1BE4] (ab110320)
![Flow Cytometry - Aconitase 2 antibody [6D1BE4] (ab110320)](/ps/datasheet/images/110/ab110320/Aconitase-2-Primary-antibodies-ab110320-3.jpg)
ab110320 at 1µg/ml staining Aconitase 2 in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).
References for Anti-Aconitase 2 antibody [6D1BE4] (ab110320)
ab110320 has not yet been referenced specifically in any publications.
Publishing research using ab110320? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"