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MitoSciences (MS793)

Anti-Aconitase 2 antibody [6F12BD9] (ab110321)

Abreviews (2)Q&A (1)

Overview

  • Product nameAnti-Aconitase 2 antibody [6F12BD9]See all Aconitase 2 primary antibodies ...
  • Description
    Mouse monoclonal [6F12BD9] to Aconitase 2
  • Tested applicationsIP, ICC/IF, IHC-Fr, WB, In-Cell ELISA, Flow Cyt, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Bovine heart mitochondria

  • Positive controlHepG2, HeLa, H9C2, and MEF cell lysates; HDFn cells and HeLa cells. Human Normal colon FFPE tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab110321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP IP: Use at an assay dependent dilution.
ICC/IF ICC/IF: Use a concentration of 1 µg/ml. for 2 hours.
IHC-Fr IHC-Fr: 1/1000.
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa.
In-Cell ELISA In-Cell ELISA: Use a concentration of 1 µg/ml.
Flow Cyt Flow Cyt: Use a concentration of 1 µg/ml.
IHC-P IHC-P: Use a concentration of 5 µg/ml.

Target

  • Alternative names
      ACO 2 antibodyACO2 antibodyACON_HUMAN antibody
      aconitase 2 antibodyAconitase 2 mitochondrial antibodyAconitase antibodyAconitase2 antibodyAconitate hydratase antibodyAconitate hydratase mitochondrial antibodyACONM antibodyCitrate hydro lyase antibodyCitrate hydro-lyase antibodymitochondrial antibody
    see all

Anti-Aconitase 2 antibody [6F12BD9] images

  • Immunocytochemistry - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)
    Immunocytochemistry - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)
    ab110321 at 1µg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
  • Flow Cytometry - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)
    Flow Cytometry - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)
    ab110321 at 1µg/ml staining Aconitase 2 in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).
  • Western blot - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)
    Western blot - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)
    All lanes : Anti-Aconitase 2 antibody [6F12BD9] (ab110321) at 1 µg/ml

    Lane 1 : HepG2 (human)
    Lane 2 : HeLa (human)
    Lane 3 : H9C2 (rat cardiomyocyte)
    Lane 4 : MEF (mouse fibroblast)

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 85 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)

    IHC image of Aconitase 2 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110321, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Frozen sections) - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)
    Immunohistochemistry (Frozen sections) - Anti-Aconitase 2 antibody [6F12BD9] (ab110321)Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
    IHC-Fr image of Aconitase 2 staining on six month old rat muscle tissue using ab110321(1:1000).4% PFA fixed sections were incubated in 0.3% PBS-Tween for 1h. The sections were then incubated with ab110321 (1:1000) overnight at +4°C. The secondary antibody Alexa Fluor® 568 donkey anti-mouse was used at a 1/1000 dilution for 1h. DAPI was used at 1.43µM

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References for Anti-Aconitase 2 antibody [6F12BD9] (ab110321)

ab110321 has not yet been referenced specifically in any publications.

Publishing research using ab110321 ? Please let us know so that we can cite the reference in this datasheet

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Western blot abreview for Anti-Aconitase 2 antibody [6F12BD9]

 Excellent
Abreviews
Application Western blot
Sample Mouse Tissue lysate - other (Brain mitochondria lysate)
Loading amount 2 µg
Specification Brain mitochondria lysate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted May 14 2012

Immunohistochemistry (Frozen sections) abreview for Anti-Aconitase 2 antibody [6F12BD9]

 Excellent
Abreviews
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Skeletal muscles)
Specification Skeletal muscles
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
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Submitted May 11 2012

Hello, I'm on the search for an antibody against aconitase2 that can be used for IP in MEF cells, and I see that you have two possible candidates, ab110321 and ab110320. I'm wondering if there's any major differences between these two, and which you would...

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Thank you for contacting us. Both ACO2 antibodies cross-react with mouse, however ab110320 is more suitable for immunoprecipitation. Ab110321 is a good WB antibody. In this particular case, I would recommend using ab110320 then. I hope this information is...

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