MitoSciences (MS793)

Anti-Aconitase 2 antibody [6F12BD9] (ab110321)

Overview

  • Product nameAnti-Aconitase 2 antibody [6F12BD9]See all Aconitase 2 primary antibodies ...
  • Description
    Mouse monoclonal [6F12BD9] to Aconitase 2
  • Tested applicationsIP, ICC/IF, IHC-Fr, WB, In-Cell ELISA, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Bovine heart mitochondria

  • Positive control
    • HepG2, HeLa, H9C2, and MEF cell lysates; HDFn cells and HeLa cells. Human Normal colon FFPE tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab110321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 µg/ml. for 2 hours.
IHC-Fr 1/1000.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

Anti-Aconitase 2 antibody [6F12BD9] images

  • ab110321 at 1µg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
  • ab110321 at 1µg/ml staining Aconitase 2 in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).
  • All lanes : Anti-Aconitase 2 antibody [6F12BD9] (ab110321) at 1 µg/ml

    Lane 1 : HepG2 (human)
    Lane 2 : HeLa (human)
    Lane 3 : H9C2 (rat cardiomyocyte)
    Lane 4 : MEF (mouse fibroblast)

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 85 kDa
  • IHC image of Aconitase 2 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110321, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC-Fr image of Aconitase 2 staining on six month old rat muscle tissue using ab110321(1:1000).4% PFA fixed sections were incubated in 0.3% PBS-Tween for 1h. The sections were then incubated with ab110321 (1:1000) overnight at +4°C. The secondary antibody Alexa Fluor® 568 donkey anti-mouse was used at a 1/1000 dilution for 1h. DAPI was used at 1.43µM

    See Abreview

References for Anti-Aconitase 2 antibody [6F12BD9] (ab110321)

This product has been referenced in:
  • Johnson MA  et al. Amino acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis. PLoS One 9:e93597 (2014). WB ; Human . Read more (PubMed: 24718614) »
  • Kovárová N  et al. High molecular weight forms of mammalian respiratory chain complex II. PLoS One 8:e71869 (2013). WB ; Human . Read more (PubMed: 23967256) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - other (Brain mitochondria lysate)
Loading amount 2 µg
Specification Brain mitochondria lysate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted May 14 2012

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Skeletal muscles)
Specification Skeletal muscles
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted May 11 2012

Thank you for contacting us. Both ACO2 antibodies cross-react with mouse, however ab110320 is more suitable for immunoprecipitation. Ab110321 is a good WB antibody. In this particular case, I would recommend using ab110320 then. I hope this information...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"