Anti-Aconitase 2 antibody (Biotin) (ab214777)

Overview

  • Product name
    Anti-Aconitase 2 antibody (Biotin)
    See all Aconitase 2 primary antibodies
  • Description
    Goat polyclonal to Aconitase 2 (Biotin)
  • Host species
    Goat
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Aconitase 2 aa 554-566 (internal sequence) (Cysteine residue). NP_001089.1
    Sequence:

    QHVDVSPTSQRLQ


    Database link: Q99798

  • Positive control
    • Human heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab214777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).

Target

Images

  • Anti-Arg2 antibody (ab21777) at 0.1 µg/ml + Human heart lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 85 kDa
    Observed band size: 90 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour and detection by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

References

ab214777 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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