RabMAb

Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

Overview

  • Product nameAnti-Aconitase 2 antibody [EPR8283(B)]
    See all Aconitase 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR8283(B)] to Aconitase 2
  • Tested applicationsICC/IF, WB, IHC-P, ICC, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the Human Aconitase 2.

  • Positive control
    • HeLa, K562, Jurkat and 293T cell lysates; Human kidney tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab129105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.
IHC-Fr 1/500.
  • Application notesIs unsuitable for IP.
  • Target

    Anti-Aconitase 2 antibody [EPR8283(B)] images

    • All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 85 kDa
    • ab129105, at 1/100 dilution, staining Aconitase 2 in Paraffin-embedded Human kidney tissue by immunohistochemistry.
    • All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/1000 dilution

      Lane 1 : 1 month old Liver Mitochondrial fraction
      Lane 2 : 3 month old Liver Mitochondrial fraction
      Lane 3 : 3 month old Liver Mitochondrial fraction
      Lane 4 : 6 month old Liver Mitochondrial fraction

      Secondary
      Pig Polyclonal to anti-Rabbit (HRP) at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 85 kDa


      Exposure time : 1 minute

      Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

      See Abreview

    • IHC-Fr image of Aconitase 2 staining of six month old rat skeletal muscles using ab129105 (1:500). 4% PFA fixed sections were incubated in 0.3% PBS-Tween for 1h. The sections were then incubated with the antibody (ab129105) overnight at +4°C. The secondary antibody Alexa Fluor® 488 donkey anti-rabbit (1:1000) for 1h. DAPI was used at a concentration of 1.43µM.

      See Abreview

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

    ab129105 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Liver)
    Specification Liver
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.3% TritonX in 0.1% PBS
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Dec 11 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Skeletal muscle)
    Specification Skeletal muscle
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.3% TritonX in 0.1% PBS
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted May 15 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - other (Liver Mitochondrial fraction)
    Loading amount 20 µg
    Specification Liver Mitochondrial fraction
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted May 15 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"