This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Liver; Human Kidney; Human Heart; Human Heart Mitochondrial; Mouse Brain; Rat Brain.
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Western blot - Anti-Aconitase 2 antibody (ab129957)
All lanes : Anti-Aconitase 2 antibody (ab129957) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Human liver tissue lysate - total protein (ab29889) Lane 3 : Human kidney tissue lysate - total protein (ab30203) Lane 4 : Human heart tissue lysate - total protein (ab29431) Lane 5 : Human Heart Mitochondrial Lysate Lane 6 : Brain (Mouse) Tissue Lysate Lane 7 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 85 kDa Observed band size : 85 kDa Additional bands at : 35 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Metodiev MD et al. Mutations in the tricarboxylic acid cycle enzyme, aconitase 2, cause either isolated or syndromic optic neuropathy with encephalopathy and cerebellar atrophy. J Med Genet51:834-8 (2014).
Read more (PubMed: 25351951) »