Overview

  • Product name
  • Description
    Rabbit polyclonal to Aconitase 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Rabbit, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human Aconitase 2.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Liver; Human Kidney; Human Heart; Human Heart Mitochondrial; Mouse Brain; Rat Brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab129957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).

Target

Images

  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Aconitase 2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab129957 observed at 85 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab129957 was shown to recognize Aconitase 2 in wild-type HAP1 cells as signal was lost at the expected MW in Aconitase 2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Aconitase 2 knockout samples were subjected to SDS-PAGE. ab129957 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Aconitase 2 antibody (ab129957) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : Human heart tissue lysate - total protein (ab29431)
    Lane 5 : Human Heart Mitochondrial Lysate
    Lane 6 : Brain (Mouse) Tissue Lysate
    Lane 7 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 85 kDa
    Observed band size: 85 kDa
    Additional bands at: 35 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129957 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:
  • Metodiev MD  et al. Mutations in the tricarboxylic acid cycle enzyme, aconitase 2, cause either isolated or syndromic optic neuropathy with encephalopathy and cerebellar atrophy. J Med Genet 51:834-8 (2014). Read more (PubMed: 25351951) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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