Overview

  • Product name
  • Description
    Rabbit polyclonal to Aconitase 2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 302-332 of Human Aconitase 2.

  • Positive control
    • 293 cell lysates, transiently transfected with the Aconitase 2 gene; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab135806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 85 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
  • Pathway
    Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
  • Sequence similarities
    Belongs to the aconitase/IPM isomerase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACO 2 antibody
    • ACO2 antibody
    • ACON_HUMAN antibody
    • aconitase 2 antibody
    • Aconitase 2 mitochondrial antibody
    • Aconitase antibody
    • Aconitase2 antibody
    • Aconitate hydratase antibody
    • Aconitate hydratase mitochondrial antibody
    • ACONM antibody
    • Citrate hydro lyase antibody
    • Citrate hydro-lyase antibody
    • ICRD antibody
    • mitochondrial antibody
    see all

Images

  • All lanes : Anti-Aconitase 2 antibody (ab135806) at 1/50 dilution

    Lane 1 : 293 cell lysates, non-transfected
    Lane 2 : 293 cell lysates, transiently transfected with the Aconitase 2 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 85 kDa
  • Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue labelling Aconitase 2 with ab135806 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

References

ab135806 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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