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Rabbit polyclonal to Aconitase 2
Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 302-332 of Human Aconitase 2.
293 cell lysates, transiently transfected with the Aconitase 2 gene; Human breast carcinoma tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Ammonium Sulphate Precipitation
ab135806 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 85 kDa.
1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.
Belongs to the aconitase/IPM isomerase family.
Information by UniProt
ACO 2 antibody
Western blot - Anti-Aconitase 2 antibody (ab135806)
All lanes : Anti-Aconitase 2 antibody (ab135806) at 1/50 dilution Lane 1 : 293 cell lysates, non-transfected Lane 2 : 293 cell lysates, transiently transfected with the Aconitase 2 gene Lysates/proteins at 2 µg per lane. Predicted band size : 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody (ab135806)
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue labelling Aconitase 2 with ab135806 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
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