Overview

  • Product name
  • Description
    Rabbit polyclonal to Aconitase 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Xenopus laevis, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Aconitase 2 (internal sequence). The exact sequence is proprietary.
    Database link: Q99798

  • Positive control
    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; Rat brain extract; Mouse brain lysate; Jurkat whole cell lysate. IP: HeLa whole cell extract. IHC-P: Mouse liver, kidney, urinary bladder and intestine tissues. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/20000. Predicted molecular weight: 85 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-Aconitase 2 antibody (ab228923) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 85 kDa



    7.5% SDS-PAGE gel.

  • Anti-Aconitase 2 antibody (ab228923) at 1/10000 dilution + Rat brain extract at 50 µg

    Predicted band size: 85 kDa



    7.5% SDS-PAGE gel.

  • Anti-Aconitase 2 antibody (ab228923) at 1/10000 dilution + Mouse brain lysate at 20 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 85 kDa



    7.5% SDS-PAGE gel.

  • Anti-Aconitase 2 antibody (ab228923) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 85 kDa



    7.5% SDS-PAGE gel.

  • Aconitase 2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab228923. Western blot was performed from the immunoprecipitate using ab228923. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG IP in HeLa whole cell extract.

    Lane 2: ab228923 IP in HeLa whole cell extract.

  • Paraffin-embedded mouse liver tissue stained for Aconitase 2 using ab228923 at 1/500 dilution in immunohistochemical analysis.

  • 2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Aconitase 2 (green) using ab228923 at 1/1000 dilution in ICC/IF.

    Red: A mitochondria tracker.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded mouse kidney tissue stained for Aconitase 2 using ab228923 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse urinary bladder tissue stained for Aconitase 2 using ab228923 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse intestine tissue stained for Aconitase 2 using ab228923 at 1/500 dilution in immunohistochemical analysis.

References

ab228923 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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