Overview

  • Product name
  • Description
    Goat polyclonal to Aconitase 2
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-QHVDVSPTSQRLQ

    , corresponding to internal sequence amino acids 554-566 of Human Aconitase 2 (NP_001089.1).

  • Positive control
    • Human Heart lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab99467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 85 kDa.

Target

Images

  • Anti-Aconitase 2 antibody (ab99467) at 0.01 µg/ml + Human Heart lysates at 35 µg

    Predicted band size: 85 kDa

  • ab99467 (3.8µg/ml) staining of paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

This product has been referenced in:
  • Márquez-Jurado S  et al. Mitochondrial levels determine variability in cell death by modulating apoptotic gene expression. Nat Commun 9:389 (2018). Read more (PubMed: 29374163) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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