Aconitase Assay Kit (ab83459)

How specific the kit is?
What is the discount for purchase of 10 kits?

ANSWER:

Thank you for contacting us.
I had chat with the product manager of this assay kit and would like to confirm that we are happy to provide you 100% Abreview discount code for this product. The discount code will be, worth the price you pay for ab83459. In brief, the procedure to avail the discount is as follows.
- Confirm to me if you are interested in the discount offer, I will then create a discount code for you.
- Buy the kit ab83459 at full price
- Send me all the experimental details and results that we can publish online; any sensitive information will be omitted
- I will process the Abreview and will validate the discount code.
- The valid discount code can be used to get a free kit or can be used to save similar value from any other purchase.
I am hoping that this kit will be a perfect fit with your samples. Once you try the first one and happy with the results, I will then send you a discounted quote for purchase of 8-10 kits.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
http://www.abcam.com/abreviews

The customer kindly phoned to ask about differences between these two products. They are working with duodenum tissues which are very active in iron absorption and are wondering if this high iron contenct will affect the activity assay as well.

ANSWER:

Thank you for contacting us.

I have been in touch with the labs regarding use of ab109712 &ab83459 for duodenumtissue because of its iron aborbence.

In regards to ab109712:Although we have not tested in this tissue type, there have been studies on aconitase and duodenal iron – one paper shows no effect.http://www.ncbi.nlm.nih.gov/pubmed/8541321

In other studies:http://www.ncbi.nlm.nih.gov/pubmed?term=iron%20dudenum%20aconitase Ppresumably the iron is sequestered after absorption preventing significant damage.



In regards to ab83459:We do not think this will be a problem, but we have not tested duodenum.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Hi, Can your aconitase kit be used on frozen tissue? Thanks! Kind Regards  

ANSWER:

Thank you for your patient.

There are two kits in our catalogue targeting Aconitase: ab83459 and ab109712. Both are compatible with frozen tissues, as long as they are frozen appropriately preserving the enzymatic activity.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

I want to detect the activity of bacteria's Aconitase, whether your product Aconitase Assay Kit suit the bacteria?

ANSWER:

As far as we are aware, this kit has never been tested with samples from bacteria. It might be possible, as the assay allows detection of c-aconitase and m-aconitase (from mitochondria), however this can not be guaranteed as it has not been tested. Maybe Walden et al (From bacteria to mitochondria: Aconitase yields surprises) can be of help.

cysteine and iron-sulfur protein amount/concentration? want to use own lysis buffer instead of assay buffer?

For 1X Cell lysis buffer: - 20mM Tris-Hcl - 150mM NaCl - 1mM Na2EDTA - 1mM EGTA - 1% Triton - 2.5mM sodium pyrophosphate - 1mM beta-glycerophosphate - 1mM Na3VO4 - 1ug/ml leupeptin

ANSWER:

The assay buffer is Tris based with some nonionic detergent. We are unable to be more specific. Other components included in your lysis buffer have not been tested for compatibility with this kit.

In regards to the content of asked components:

Cysteine - 2mg/ kit (NH4)2Fe(SO4)2 - 40 ug/ kit