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Overview

  • Product nameAconitase Assay KitSee all Aconitase kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Assay time
    0h 40m
  • Product overview

    Abcam's Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λmax at 450nm.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceAconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with prooxidants has been interpreted as a measure of oxidative damage.
  • Cellular localizationACO1: Cytoplasmic ACO2: Mitochondrial
    • Alternative names
        ACO 1ACO 2ACO1
        ACO2Aconitase 1 solubleAconitase 2 mitochondrialAconitate hydrataseAconitate hydratase mitochondrialACONMACONSCitrate hydro lyaseCytoplasmic aconitate hydrataseEC 4.2.1.3Ferritin repressor proteinIRE BP 1IREB1IREBPIREBP1Iron regulatory protein 1Iron responsive element binding protein 1IRP1MGC20605MGC33908
      see all

    Applications

    Our Abpromise guarantee covers the use of ab83459 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Aconitase Assay Kit images

    • Functional Studies - Aconitase Assay Kit (ab83459)
      Functional Studies - Aconitase Assay Kit (ab83459)
      Isocitrate standard curve using ab83459.
    • Functional Studies - Aconitase Assay Kit (ab83459)
      Functional Studies - Aconitase Assay Kit (ab83459)
      30 minute Aconitase sample test using ab83459.

    Protocols

    References for Aconitase Assay Kit (ab83459)

    This product has been referenced in:
    • Nishiki Y  et al. Translational control of inducible nitric oxide synthase by p38 MAPK in islet ß-cells. Mol Endocrinol 27:336-49 (2013). Read more (PubMed: 23250488) »

    See 1 Publication for this product

    Publishing research using ab83459 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab83459 Submit a Question for ab83459

    Displaying 1 - 9 of 9 results for Abreviews and Q&A

    10
    Votes: Highest First

    To whom it may concern,
    I have purchased 3 assay kits, which I would like to inquire
    further details about the products which however are not thoroughly
    indicated in the user manual. I would appreciate for your feedback on
    that:

    Read More

    Thank you for contacting us.

    I have received word back from the lab regarding your request. Unfortunately the details of the reagents and reactions for these products are proprietary and we cannot share them. They have provided what details o...

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    Hello!
    Thank you for the reply. I mean the aconitase kit ab83459. There is a formula in the section of data analysis.
    Good thing about this kit is that a little amount of tissue is necessary for analysis, in my case it would be brain tissue and ...

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    Thank you for your interest in our products.

    I have been discussing your enquiry with my colleagues in the Lab and would like to share the following information:

    Regarding ab109712: Aconitase Enzyme Activity Microplate Assay Kit
    <...

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    I wonder if the ABCAM company sells kits to dosage of the enzymes citrate synthase, aconitase, citrate lyase, NAD + and NADH isocitrate dehydrogenase and isocitrate lyase activitys.

    Thank you for contacting us. You may be interested in the following kits:

    Citrate Synthase: ab119692
    Aconitase: ab109712 or ab83459
    CitrateLyase: wedo not currently have a kit available, but weyou may be interested in ourprimary antibo...

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    How specific the kit is?
    What is the discount for purchase of 10 kits?

    Thank you for contacting us.
    I had chat with the product manager of this assay kit and would like to confirm that we are happy to provide you 100% Abreview discount code for this product. The discount code will be, worth the price you pay for ab83459...

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    The customer kindly phoned to ask about differences between these two products. They are working with duodenum tissues which are very active in iron absorption and are wondering if this high iron contenct will affect the activity assay as well.

    Thank you for contacting us.

    I have been in touch with the labs regarding use of ab109712 &ab83459 for duodenumtissue because of its iron aborbence.

    In regards to ab109712:Although we have not tested in this tissue type, there ...

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    Hi, Can your aconitase kit be used on frozen tissue? Thanks! Kind Regards  

    Thank you for your patient. There are two kits in our catalogue targeting Aconitase: ab83459 and ab109712. Both are compatible with frozen tissues, as long as they are frozen appropriately preserving the enzymatic activity. I hope this infor...

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    I want to detect the activity of bacteria's Aconitase, whether your product Aconitase Assay Kit suit the bacteria?

    As far as we are aware, this kit has never been tested with samples from bacteria. It might be possible, as the assay allows detection of c-aconitase and m-aconitase (from mitochondria), however this can not be guaranteed as it has not been tested. Mayb...

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    cysteine and iron-sulfur protein amount/concentration? want to use own lysis buffer instead of assay buffer? For 1X Cell lysis buffer: - 20mM Tris-Hcl - 150mM NaCl - 1mM Na2EDTA - 1mM EGTA - 1% Triton - 2.5mM sodium pyrophospha...

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    The assay buffer is Tris based with some nonionic detergent. We are unable to be more specific. Other components included in your lysis buffer have not been tested for compatibility with this kit. In regards to the content of asked components: Cy...

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    has this antibody been tested in mouse tissue? what tissues were tested?

    The assay kit should be compatible with mammalian species. We have not tested it will all samples however we know that the kit can be adapted to samples from mammals by the researcher. Unfortunately we have no further information for you on mouse tissue.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"