Overview

  • Product nameAconitase Assay Kit
    See all Aconitase kits
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Assay time
    0h 40m
  • Product overview

    Abcam's Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λmax at 450nm.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceAconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with prooxidants has been interpreted as a measure of oxidative damage.
  • Cellular localizationACO1: Cytoplasmic ACO2: Mitochondrial
  • Alternative names
    • ACO 1
    • ACO 2
    • ACO1
    • ACO2
    • Aconitase 1 soluble
    • Aconitase 2 mitochondrial
    • Aconitate hydratase
    • Aconitate hydratase mitochondrial
    • ACONM
    • ACONS
    • Citrate hydro lyase
    • Cytoplasmic aconitate hydratase
    • EC 4.2.1.3
    • Ferritin repressor protein
    • IRE BP 1
    • IREB1
    • IREBP
    • IREBP1
    • Iron regulatory protein 1
    • Iron responsive element binding protein 1
    • IRP1
    • MGC20605
    • MGC33908
    see all

Applications

Our Abpromise guarantee covers the use of ab83459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Aconitase Assay Kit images

  • Aconitase measured in mouse tissue lysates showing quantity (mU) per mg of tested sample.
  • 30 minute Aconitase sample test using ab83459.
  • Isocitrate standard curve using ab83459.

Protocols

References for Aconitase Assay Kit (ab83459)

This product has been referenced in:
  • Nishiki Y  et al. Translational control of inducible nitric oxide synthase by p38 MAPK in islet ß-cells. Mol Endocrinol 27:336-49 (2013). Read more (PubMed: 23250488) »
  • Cummins N  et al. Changes to mitochondrial ultrastructure in optic nerve vulnerable to secondary degeneration in vivo are limited by irradiation at 670 nm. BMC Neurosci 14:98 (2013). Read more (PubMed: 24011177) »

See all 2 Publications for this product

Product Wall

The mU/ml shows activity of Aconitase per unit volume. Another way of expressing the numbers will be if you perform a protein quantitation assay and instead of the ml volume of sample, uses mg of total protein. In this scenario the final numbers can be...

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For ab83459, we have optimized this assay protocol for samples of mammalian origin. However, theoretically this assay should work with samples from multiple origins including bacteria. Since we have not done it ourselves, I do not know if the assay pro...

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Thank you for contacting us.

I have received word back from the lab regarding your request. Unfortunately the details of the reagents and reactions for these products are proprietary and we cannot share them. They have provided what detail...

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Thank you for your interest in our products.

I have been discussing your enquiry with my colleagues in the Lab and would like to share the following information:

Regarding ab109712: Aconitase Enzyme Activity Microplate Assay Kit Read More

Thank you for contacting us. You may be interested in the following kits:

Citrate Synthase: ab119692
Aconitase: ab109712 or ab83459
CitrateLyase: wedo not currently have a kit available, but weyou may be interested in ourprimary ant...

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Thank you for contacting us.
I had chat with the product manager of this assay kit and would like to confirm that we are happy to provide you 100% Abreview discount code for this product. The discount code will be, worth the price you pay for ab83...

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Thank you for contacting us.

I have been in touch with the labs regarding use of ab109712 &ab83459 for duodenumtissue because of its iron aborbence.

In regards to ab109712:Although we have not tested in this tissue type, the...

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Thank you for your patient. There are two kits in our catalogue targeting Aconitase: ab83459 and ab109712. Both are compatible with frozen tissues, as long as they are frozen appropriately preserving the enzymatic activity. I hope this in...

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As far as we are aware, this kit has never been tested with samples from bacteria. It might be possible, as the assay allows detection of c-aconitase and m-aconitase (from mitochondria), however this can not be guaranteed as it has not been tested. M...

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The assay buffer is Tris based with some nonionic detergent. We are unable to be more specific. Other components included in your lysis buffer have not been tested for compatibility with this kit. In regards to the content of asked components: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"