Aconitase Assay Kit (ab83459)
Overview
- Product nameAconitase Assay KitSee all Aconitase kits ...
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeEnzyme activity
- Assay time0h 40m
- Product overview
Abcam's Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λmax at 450nm.
Visit our FAQs page for tips and troubleshooting. - Notes
Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage.
- Tested applicationsFunctional Studies more details
Properties
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Components Identifier 100 tests (NH4)2Fe(SO4)2 (Lyophilised) Brown/NM 1 vial Assay Buffer WM 1 x 30ml Cysteine (Lyophilised) Red 1 vial Developer (Lyophilised) Purple 1 vial Enzyme Mix Green 1 x 200µl Isocitrate Standard (10µmol) (lyophilized) Yellow 1 vial Substrate (Lyophilised) Blue 1 vial - Research Areas
- RelevanceAconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with prooxidants has been interpreted as a measure of oxidative damage.
- Cellular localizationACO1: Cytoplasmic ACO2: Mitochondrial
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Alternative names
- ACO 1
- ACO 2
- ACO1
- ACO2
- Aconitase 1 soluble
- Aconitase 2 mitochondrial
- Aconitate hydratase
- Aconitate hydratase mitochondrial
- ACONM
- ACONS
- Citrate hydro lyase
- Cytoplasmic aconitate hydratase
- EC 4.2.1.3
- Ferritin repressor protein
- IRE BP 1
- IREB1
- IREBP
- IREBP1
- Iron regulatory protein 1
- Iron responsive element binding protein 1
- IRP1
- MGC20605
- MGC33908
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Applications
Our Abpromise guarantee covers the use of ab83459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Aconitase Assay Kit images
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Functional Studies - Aconitase Assay Kit (ab83459)Isocitrate standard curve using ab83459. -
Functional Studies - Aconitase Assay Kit (ab83459)30 minute Aconitase sample test using ab83459.
Protocols
References for Aconitase Assay Kit (ab83459)
This product has been referenced in:
- Nishiki Y et al. Translational control of inducible nitric oxide synthase by p38 MAPK in islet ß-cells. Mol Endocrinol 27:336-49 (2013). Read more (PubMed: 23250488) »
- Cummins N et al. Changes to mitochondrial ultrastructure in optic nerve vulnerable to secondary degeneration in vivo are limited by irradiation at 670 nm. BMC Neurosci 14:98 (2013). Read more (PubMed: 24011177) »
