|
|
|
|
Functional Studies - Aconitase Assay Kit (ab83459)
(enlarge)
|
|
Functional Studies - Aconitase Assay Kit (ab83459)
(enlarge)
|
|
|
|
|
Product Name
|
Aconitase Assay Kit
|
|
Product type
|
Kits
|
|
Description
|
Aconitase Assay Kit
|
|
Research areas
|
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
Signal Transduction >> Metabolism >> Energy Metabolism
|
|
Product overview
|
The Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a Lambda max at 450nm.
|
|
Components
|
| Kit Components | Unit Size/Volume | Cap Colour |
|---|
| Assay Buffer | 30ml | WM |
| Substrate | lyophilized | Blue | | Developer | lyophilized | Purple | | Enzyme Mix | 200 µl | Green | | Cysteine | lyophilized | Red | | (NH4)2Fe(SO4)2 | lyophilized | Brown | | Isocitrate Standard (10µmol) | lyophilized | Yellow |
Note: Please Click Here for Instructions For Use/Protocol
|
|
Storage instructions
|
Store at -20°C. Stable for 12 months at -20°C
|
|
Tested applications
(see key)
|
FuncS
|
|
Notes
|
Protect kit from light. Warm the assay buffer to room temperature before use. Briefly centrifuge vials before opening. Keep samples, Enzyme Mix and Aconitase solution on ice during the assay.
Aconitase Activation Solution: Store reconstituted cysteine and (NH4)2Fe(SO4)2 at -20°C. Take out 0.1ml cysteine and (NH4)2Fe(SO4)2 solutions and mix together to prepare fresh activation solution.
All the reconstituted solutions except the activated Aconitase are stable for 1 month at -20°C.
|
|
|
Material safety datasheet (MSDS) for this product:
Aconitase Assay Kit MSDS
|
|
Relevance
|
Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with prooxidants has been interpreted as a measure of oxidative damage.
|
|
|
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Aconitase Assay Kit is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
Aconitase Assay Kit images:
|
 Functional Studies - Aconitase Assay Kit (ab83459)
Isocitrate standard curve using ab83459.
|
| |
Functional Studies - Aconitase Assay Kit (ab83459)
30 minute Aconitase sample test using ab83459.
|
| |
| |
|
Search PubMed (MEDLINE) for references to Aconitase
|
| |
Aconitase Assay Kit - more information
|
Customer reviews (feedback) regarding Aconitase Assay Kit |
Customer FAQs regarding Aconitase Assay Kit |
Protocols for Aconitase Assay Kit |
Price and availability of products related to Aconitase Assay Kit |
| |
Aconitase Assay Kit - other tools:
|
Contact Abcam with a Technical Enquiry about Aconitase Assay Kit |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
