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Products:Signal Transduction >> Metabolism >> Energy Metabolism
Overview
Product name
Aconitase Assay Kit
See all Aconitase products (2) ...
Product overview
The Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a Lambda max at 450nm.
Notes
Protect kit from light. Warm the assay buffer to room temperature before use. Briefly centrifuge vials before opening. Keep samples, Enzyme Mix and Aconitase solution on ice during the assay.
Aconitase Activation Solution: Store reconstituted cysteine and (NH4)2Fe(SO4)2 at -20°C. Take out 0.1ml cysteine and (NH4)2Fe(SO4)2 solutions and mix together to prepare fresh activation solution.
All the reconstituted solutions except the activated Aconitase are stable for 1 month at -20°C.
Aconitase Activation Solution: Store reconstituted cysteine and (NH4)2Fe(SO4)2 at -20°C. Take out 0.1ml cysteine and (NH4)2Fe(SO4)2 solutions and mix together to prepare fresh activation solution.
All the reconstituted solutions except the activated Aconitase are stable for 1 month at -20°C.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
| Components | Identifier | 100 tests |
|---|---|---|
| (NH4)2Fe(SO4)2 (Lyophilised) | Brown/NM | 1000 vials |
| Assay Buffer | WM | 1 x 30ml |
| Cysteine (Lyophilised) | Red | 1 vial |
| Developer (Lyophilised) | Purple | 1 vial |
| Enzyme Mix | Green | 1 x 200µl |
| Isocitrate Standard (10µmol) (lyophilized) | Yellow | 1 vial |
| Substrate (Lyophilised) | Blue | 1 vial |
Research areas
Kits/ Lysates/ Other >> Kits >> Cell Damage Kits >> Oxidative stress kits
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Oxidative Stress Assay Kits
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
Signal Transduction >> Metabolism >> Energy Metabolism
Relevance
Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with prooxidants has been interpreted as a measure of oxidative damage.
Cellular localization
ACO1: Cytoplasmic ACO2: Mitochondrial
Alternative names
- ACO 1
- ACO 2
- ACO1
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab83459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use at an assay dependent dilution.
Aconitase Assay Kit images:
Protocols
References for Aconitase Assay Kit (ab83459)
ab83459 has not yet been referenced specifically in any publications.
Publishing research using ab83459? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"