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Aconitase Assay Kit
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The Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a Lambda max at 450nm.
Functional Studiesmore details
Store at -20°C. Stable for 12 months at -20°C
| Components | Identifier | 100 tests |
|---|---|---|
| (NH4)2Fe(SO4)2 (Lyophilised) | Brown/NM | 1000 vials |
| Assay Buffer | WM | 1 x 30ml |
| Cysteine (Lyophilised) | Red | 1 vial |
| Developer (Lyophilised) | Purple | 1 vial |
| Enzyme Mix | Green | 1 x 200µl |
| Isocitrate Standard (10µmol) (lyophilized) | Yellow | 1 vial |
| Substrate (Lyophilised) | Blue | 1 vial |
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Signal Transduction >> Metabolism >> Energy Metabolism
Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with prooxidants has been interpreted as a measure of oxidative damage.
ACO1: Cytoplasmic ACO2: Mitochondrial
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use at an assay dependent dilution.
ab83459 has not yet been referenced specifically in any publications.
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