Anti-Acrolein (conjugated) antibody (ab37110)

Overview

  • Product nameAnti-Acrolein (conjugated) antibody
    See all Acrolein primary antibodies
  • Description
    Rabbit polyclonal to Acrolein (conjugated)
  • SpecificityThis antibody targets conjugated acrolein. This antibody does not recognize free acrolein.

    COMPOUND : CROSS REACTIVITY
    Acrolein-BSA : 1
    Malondialdehyde-BSA : 1/55
    Glyoxal-BSA : 1/>50,000
    Formaldehyde-BSA : 1/>50,000
    Glutaraldehyde-BSA : 1/>50,000
    BSA = Bovine Serum Albumin
    Acrolein-BSA concentration/unconjugated or conjugated analogs concentration at half displacement.
  • Tested applicationsELISA, IHC-Fr, IHC-Pmore details
  • Immunogen

    Chemical/ Small Molecule conjugated to BSA

Properties

Applications

Our Abpromise guarantee covers the use of ab37110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/5000.
IHC-Fr 1/500 - 1/1000. (cryostat)
IHC-P Use at an assay dependent concentration. PubMed: 21953681

Target

  • RelevanceAcrolein, a reactive alpha,beta-unsaturated aldehyde, is a common environmental pollutant, a metabolite of the anticancer drug cyclophosphamide, and a byproduct of lipid peroxidation. An increase in acrolein production has been proposed as a marker for Alzheimer's disease, diabetic glomerular lesions, and atherosclerosis. Acrolein is a potent inhibitor of cell proliferation at nonlethal doses and may act through effects on redox-regulated transcription factors. It has been reported that NF-kappaB activation is inhibited by acrolein.
  • Alternative names
    • 2-Propen-1-one antibody
    • 2-propenal antibody
    • Acraldehyde antibody
    • Acrolein antibody
    • Acrylic aldehyde antibody
    see all

References for Anti-Acrolein (conjugated) antibody (ab37110)

This product has been referenced in:
  • Shearn CT  et al. Identification of 5' AMP-activated Kinase as a Target of Reactive Aldehydes during Chronic Ingestion of High Concentrations of Ethanol. J Biol Chem 289:15449-15462 (2014). Read more (PubMed: 24722988) »
  • Spiess PC  et al. Acrolein exposure suppresses antigen-induced pulmonary inflammation. Respir Res 14:107 (2013). IHC-P . Read more (PubMed: 24131734) »

See all 7 Publications for this product

Product Wall

Thank you for your inquiry. This antibody is not conjugated, the chemical which was used as the immunogen was conjugated with BSA. So you can use HRP conjugated second antibody.

Thank you for your reply.

I confirmed with the lab that this assay was done using the same ELISA protocol that we had attached to the earlier email (CCE 3753830). I've attached it again for your reference.

I hope this information helps.

Thank you for your reply,

I've attached the ELISA protocol that is recommended with this antibody.

This product is designed to detect acrolein in the presence of gluteraldehyde.


I hope this information helps. Please...

Read More

Thank you for contacting Abcam regarding ab37110.


This antibody will NOT recognize free acrolein. Only acrolein in the presence of gluteraldehyde. The ELISA performed to QC this antibody and test cross-reactivity was performed with the ...

Read More
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (retina)
Specification retina
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"