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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Overview
Product name
Anti-Acrosin antibody [ACR-2]
See all Acrosin products (3) ...
Description
Mouse monoclonal [ACR-2] to Acrosin
Specificity
The antibody ACR 2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa.
Tested applications
ICC, WBmore details
Cross reactivity
Reacts with
Pig
Does not react with
Cow, Dog, Human
Immunogen
Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified from ascites by protein A affinity chromatography.
Clonality
Monoclonal
Clone number
ACR-2
Isotype
IgG1
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC
WB
Application notes
ICC: Use at an assay dependent dilution membrane permeabilization (acetone) essential.
WB: Use at an assay dependent dilution.
The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.
Alternative names
- ACR antibody
- Acrosin precursor antibody
- ACRS antibody
see all
Database links
References for Anti-Acrosin antibody [ACR-2] (ab1900)
This product has been referenced in:
- Moos Jet al. Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod 49:408-15 (1993).Read more (PubMed: 8373968) »
- Moos Jet al. Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction. Biochim Biophys Acta 1176:199-207 (1993).Read more (PubMed: 8471622) »
See all 4 publications for this product
Publishing research using ab1900? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"