Anti-Acrosin antibody (ab95155)

Overview

  • Product nameAnti-Acrosin antibody
    See all Acrosin primary antibodies
  • Description
    Rabbit polyclonal to Acrosin
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Acrosin aa 99-127 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P10323

  • Positive control
    • T47D cell line lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab95155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 46 kDa.

Target

  • FunctionAcrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acr antibody
    • ACRO_HUMAN antibody
    • Acrosin heavy chain antibody
    • Acrosin precursor antibody
    • ACRS antibody
    • Preproacrosin antibody
    • Proacrosin antibody
    see all

Anti-Acrosin antibody images

  • Anti-Acrosin antibody (ab95155) at 1/50 dilution + T47D cell line lysates at 35 µg/ml

    Predicted band size : 46 kDa

References for Anti-Acrosin antibody (ab95155)

ab95155 has not yet been referenced specifically in any publications.

Product Wall

The immunogen sequence of ab95155 shares 79% identity with the mouse sequence given at the UniProt entry, http://www.uniprot.org/uniprot/P23578. Cross-reaction of the antibody with mouse acrosin is possible, but unconfirmed.

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (marmoset adult testis section)
Specification marmoset adult testis section
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Zachary Yu-Ching Lin

Verified customer

Submitted Sep 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"