Anti-Acrosin antibody (ab95155)

Overview

  • Product nameAnti-Acrosin antibodySee all Acrosin primary antibodies ...
  • Description
    Rabbit polyclonal to Acrosin
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Acrosin aa 99-127 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P10323

  • Positive control
    • T47D cell line lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab95155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/50 - 1/100. Predicted molecular weight: 46 kDa.

Target

  • RelevanceAcrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.
  • Database links
  • Alternative names
    • ACR antibody
    • Acrosin precursor antibody
    • ACRS antibody
    • Preproacrosin antibody
    • Proacrosin antibody
    see all

Anti-Acrosin antibody images

  • Anti-Acrosin antibody (ab95155) at 1/50 dilution + T47D cell line lysates at 35 µg/ml

    Predicted band size : 46 kDa

References for Anti-Acrosin antibody (ab95155)

ab95155 has not yet been referenced specifically in any publications.

Product Wall

The immunogen sequence of ab95155 shares 79% identity with the mouse sequence given at the UniProt entry, http://www.uniprot.org/uniprot/P23578. Cross-reaction of the antibody with mouse acrosin is possible, but unconfirmed.

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (marmoset adult testis section)
Specification marmoset adult testis section
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Zachary Yu-Ching Lin

Verified customer

Submitted Sep 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"