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Anti-Actin antibody [1A4+5C5] (ab17146)

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Overview

  • Product nameAnti-Actin antibody [1A4+5C5]See all Actin primary antibodies ...
  • Description
    Mouse monoclonal [1A4+5C5] to Actin
  • Tested applicationsIHC-P more details
  • Species reactivity
    Reacts with: Rat, Sheep, Rabbit, Guinea pig, Cow, Human, Snake
  • Immunogen

    BALB/C mice were injected with N-terminal decapeptide of alpha-smooth muscle actin for 1A4 and with purified rabbit striated muscle actin for 5C5.

  • Positive controlSkeletal muscle

Properties

Applications

Our Abpromise guarantee covers the use of ab17146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/25 - 1/50.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • Sequence similaritiesBelongs to the actin family.
  • Cellular localizationCytoplasm > cytoskeleton.

    • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        ACTA antibodyACTA1 antibodyActin alpha skeletal muscle antibody
        Actin antibodyactin, alpha 1, skeletal muscle 1 antibodyactin, alpha 1, skeletal muscle antibodyActin, alpha skeletal muscle antibodyACTS_HUMAN antibodyalpha Actin 1 antibodyalpha skeletal muscle Actin antibodyalpha skeletal muscle antibodyAlpha-actin-1 antibodyASMA antibodyCFTD antibodyCFTD1 antibodyCFTDM antibodyMPFD antibodyNEM1 antibodyNEM2 antibodyNEM3 antibodynemaline myopathy type 3 antibody
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    References for Anti-Actin antibody [1A4+5C5] (ab17146)

    ab17146 has not yet been referenced specifically in any publications.

    Publishing research using ab17146 ? Please let us know so that we can cite the reference in this datasheet

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    We are interested in alpha cardiac actin. We are looking at rat heart homogenate with Western blot. I am confused with all the actins. Does the alpha cardiac actin you refer to go by other names in the literature? We presently use an antibody to alpha...

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    We can confirm that alpha cardiac striated muscle actin is also called sarcomeric actin. For example, Ab7799 reacts with alpha cardiac striated muscle (sarcomeric) and alpha skeletal muscle. Ab7817 reacts with alpha smooth muscle, does not react with st...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"